{"id":26566,"date":"2018-03-09T09:18:27","date_gmt":"2018-03-09T09:18:27","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/determinantes-estructurales-del-procesamiento-y-la-actividad-catala%c2%adtica-de-tirosinasa\/"},"modified":"2018-03-09T09:18:27","modified_gmt":"2018-03-09T09:18:27","slug":"determinantes-estructurales-del-procesamiento-y-la-actividad-catala%c2%adtica-de-tirosinasa","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/murcia\/determinantes-estructurales-del-procesamiento-y-la-actividad-catala%c2%adtica-de-tirosinasa\/","title":{"rendered":"Determinantes estructurales del procesamiento y la actividad catal\u00edtica de tirosinasa."},"content":{"rendered":"<h2>Tesis doctoral de <strong> M. Concepci\u00f3n Olivares S\u00e1nchez <\/strong><\/h2>\n<p>La melanog\u00e9nesis es la ruta bioqu\u00edmica responsable de la s\u00edntesis de melaninas que, en mam\u00edferos, est\u00e1 restringida a unos org\u00e1nulos espec\u00edficos de los melanocitos llamados melanosomas. Este proceso est\u00e1 calizado por al menos tres metaloenzimas que presentan una alta homolog\u00eda (tirosinasa y las tyrps, tyrp1 y tyrp2), aunque sus capacidades catal\u00edticas son diferentes, tanto entre ellas como entre distintas especies. El objetivo principal de esta tesis ha sido el estudio de las relaciones estructura-funci\u00f3n de las prote\u00ednas de la familia, especialmente de la tirosinasa, la enzima limitante de la velocidad de la ruta. En concreto, estudiamos:  i)las diferencias entre las propiedades enzim\u00e1ticas de las tirosinasas humana y de rat\u00f3n; ii) residuos del centro activo de tirosinasa que participaran en la uni\u00f3n del cofactor met\u00e1lico cobre y el reconocimiento de los sustratos; iii) papel de la glicosilaci\u00f3n de la tirosinasa. Las conclusiones m\u00e1s importantes de este estudio han sido:  1- la tirosinasa es la enzima responsable en humanos de la actividad dhica oxidasa de la ruta melanog\u00e9nica, en oposici\u00f3n a la enzima de rat\u00f3n, que es incapaz de catalizar esta reacci\u00f3n (con anterioridad se hab\u00eda demostrado que en rat\u00f3n es tyrp1).  2- se ha identificado de forma, inequ\u00edvoca el papel de dos importantes amino\u00e1cidos (his) del centro activo de la tirosinasa de rat\u00f3n: his390 es el tercer ligando del cobre en la regi\u00f3n de uni\u00f3n a metal b de la enzima, mientras qwue his389 es responsable de la interacci\u00f3n enantioespec\u00edfica de la enzima con los difenoles, pero no con los monofenoles. Esto ha propiciado la introducci\u00f3n de relevantes aportaciones al mecanismo del ciclo catal\u00edtico de tirosinasa, entre las que destaca al establecimiento de la inequiValencia de  los dos cobres del centro activo: los monofenoles se unen al cobre a y los difenoles al b.  3- la uni\u00f3n del cobre es un evento posterior en la maduraci\u00f3n post-traduccional<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Determinantes estructurales del procesamiento y la actividad catal\u00edtica de tirosinasa.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Determinantes estructurales del procesamiento y la actividad catal\u00edtica de tirosinasa. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 M. Concepci\u00f3n Olivares S\u00e1nchez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 30\/10\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>borr\u00f3n Mart\u00ednez Garc\u00eda<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal:  Lozano teruel Jos\u00e9 Antonio <\/li>\n<li>Mar\u00eda  rosario Castellar rodriguez (vocal)<\/li>\n<li>ghanem Ghanem (vocal)<\/li>\n<li>lLuis Montoliu jose (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de M. Concepci\u00f3n Olivares S\u00e1nchez La melanog\u00e9nesis es la ruta bioqu\u00edmica responsable de la s\u00edntesis de melaninas que, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[8235],"tags":[76781,8531,48557,8312,76780,76782],"class_list":["post-26566","post","type-post","status-publish","format-standard","hentry","category-murcia","tag-borron-Martinez-garcia","tag-ghanem-ghanem","tag-lluis-montoliu-jose","tag-lozano-teruel-jose-antonio","tag-m-concepcion-olivares-sanchez","tag-maria-rosario-castellar-rodriguez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/26566","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=26566"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/26566\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=26566"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=26566"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=26566"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}