{"id":26605,"date":"2018-03-09T09:18:30","date_gmt":"2018-03-09T09:18:30","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/protea%c2%adnas-de-fase-aguda-en-el-perro-estudios-de-validacion-anala%c2%adtica-y-cla%c2%adnica\/"},"modified":"2018-03-09T09:18:30","modified_gmt":"2018-03-09T09:18:30","slug":"protea%c2%adnas-de-fase-aguda-en-el-perro-estudios-de-validacion-anala%c2%adtica-y-cla%c2%adnica","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/murcia\/protea%c2%adnas-de-fase-aguda-en-el-perro-estudios-de-validacion-anala%c2%adtica-y-cla%c2%adnica\/","title":{"rendered":"Prote\u00ednas de fase aguda en el perro: estudios de validaci\u00f3n anal\u00edtica y cl\u00ednica."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Silvia Martinez Subiela <\/strong><\/h2>\n<p>En el presente trabajo se establecieron varios objetivos (metodol\u00f3gicos y de aplicaci\u00f3n cl\u00ednica) para intentar resolver algunas de las limitaciones existentes para el empleo de las determinaciones de prote\u00ednas de fase aguda en la especie canina.  como primer objetivo se validaron los an\u00e1lisis comerciales disponibles en la actualidad para la determinaci\u00f3n de haptoglobina, prote\u00edna c reacitva (crp) y amiloide a s\u00e9rico (saa) en el perro. Estas pruebas mostraron una buena precisi\u00f3n intradeterminaci\u00f3n (cvs &lt;10%) y exactitud, por tanto pueden ser empleadas en nuestro laboratorio para medir haptoglobina, prote\u00edna c reactiva y amiloide a s\u00e9rico en muestras de la especie canina.Sin embargo, los an\u00e1lisis de crp y saa mostraron una alta imprecisi\u00f3n interdeterminaci\u00f3n, por lo que en el resto de ensayos realizados las muestras se congelaron a -20% y analizaron en la misma serie anal\u00edtica.  como segundo objetivo se desarrollo y valid\u00f3 en nuestro laboratorio en nuevo m\u00e9todo automatizado basado en la capacidad oxidativa de la ceruloplasmina sobre la p-fenilenediamina para la determianci\u00f3n de esta prote\u00edna en el perro. Se determinar\u00f3n las concentraciones \u00f3ptimas de sustrato (61,5 mg en 25 ml de tamp\u00f3n), el ph \u00f3ptimo del tamp\u00f3n (5,2), los tiempos \u00f3ptimos de lectura (200-300 segundos) y el efecto del edta en la reacci\u00f3n (negativo). para la validaci\u00f3n se evalu\u00f3 la precisi\u00f3n y exactitud del m\u00e9todo, siendo ambas satisfactorias. Se comprob\u00f3 la estabilidad del reactivo (hasta 1 mes en congelaci\u00f3n,15 d\u00edas en refrigeraci\u00f3n y 7 d\u00edas a temperatura ambiente) y se estudi\u00f3 su posible aplicaci\u00f3n en muestras biol\u00f3gicas, comprob\u00e1ndose su utilidad para la detecci\u00f3n de procesos inflamatorios tales como piometra o traumatismos.  el tercer objetivo se centr\u00f3 en la evaluaci\u00f3n de los posibles efectos que la hem\u00f3lisis, lipemia,bilirrubinemia y los anticoagulantes pueden tener sobre los resultados de prote\u00edna c reactiva, amiloide<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Prote\u00ednas de fase aguda en el perro: estudios de validaci\u00f3n anal\u00edtica y cl\u00ednica.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Prote\u00ednas de fase aguda en el perro: estudios de validaci\u00f3n anal\u00edtica y cl\u00ednica. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Silvia Martinez Subiela <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 31\/10\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Joaquin Cer\u00f3n Madrigal<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: candido Guiterrez panizo <\/li>\n<li>amdreas Moritz (vocal)<\/li>\n<li> Ginel perez pedro j. (vocal)<\/li>\n<li>david Eckersall philip (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Silvia Martinez Subiela En el presente trabajo se establecieron varios objetivos (metodol\u00f3gicos y de aplicaci\u00f3n cl\u00ednica) para [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[8235],"tags":[76886,76885,76888,76887,65957,76884],"class_list":["post-26605","post","type-post","status-publish","format-standard","hentry","category-murcia","tag-amdreas-moritz","tag-candido-guiterrez-panizo","tag-david-eckersall-philip","tag-ginel-perez-pedro-j","tag-jose-joaquin-ceron-madrigal","tag-silvia-Martinez-subiela"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/26605","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=26605"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/26605\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=26605"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=26605"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=26605"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}