{"id":27441,"date":"2003-11-12T00:00:00","date_gmt":"2003-11-12T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/regulacion-de-oxido-na%c2%adtrico-sintasa-inducible-en-la-citoproteccion-por-pge1-de-la-muerte-celular-inducida-por-d-galactosamina-en-cultivo-primario-de-hepatocitos-de-rata\/"},"modified":"2003-11-12T00:00:00","modified_gmt":"2003-11-12T00:00:00","slug":"regulacion-de-oxido-na%c2%adtrico-sintasa-inducible-en-la-citoproteccion-por-pge1-de-la-muerte-celular-inducida-por-d-galactosamina-en-cultivo-primario-de-hepatocitos-de-rata","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/cordoba\/regulacion-de-oxido-na%c2%adtrico-sintasa-inducible-en-la-citoproteccion-por-pge1-de-la-muerte-celular-inducida-por-d-galactosamina-en-cultivo-primario-de-hepatocitos-de-rata\/","title":{"rendered":"Regulaci\u00f3n de \u00f3xido n\u00edtrico sintasa inducible en la citoprotecci\u00f3n por pge1 de la muerte celular inducida por d-galactosamina en cultivo primario de hepatocitos de rata"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Emilio Siendones Castillo <\/strong><\/h2>\n<p>Introducci\u00f3n  la administraci\u00f3n de d-galactosamina (d-galn) es un modelo experimental de toxicidad hep\u00e1tica adecuado debdio a su especificidad y a que reproduce el da\u00f1o hep\u00e1tico observado en hepatitis, fallo hep\u00e1tico fulminante y fallo hep\u00e1tico subfulminante. D-galactosamina bloquea la s\u00edntesis de rna y prote\u00ednas y por tanto altera las funciones hepatocelulares. Prostaglandina e (pge) ejerce un efecto protector frente a la toxicidad y muerte celular inducida en el h\u00edgado por d-galn y otros modelos de toxicidad hep\u00e1tica. Adem\u00e1s, est\u00e1 demostrado que el tratamiento de pge1 tiene efectos beneficiosos sobre el fallo hep\u00e1tico fulminante de origen viral en humanos, reduciendo los niveles de transaminasas y mejorando las condiciones de endefalopat\u00eda y tasa de protrombina.  objetivos  esta tesis forma parte de una serie de proyectos de investigaci\u00f3n que tienen como objetivo estudiar los efectos hepatoprotectores de pge1, as\u00ed como la fisiopatolgo\u00eda experimental del fallo hep\u00e1tico fulminante. El presente proyecto de tesis tuvo como objetivos estudiar la inducci\u00f3n de muerte celular por d-galn en cultivo primario en hepatocitos de rata y as\u00ed como los mecanismos de resistencia que la preadministraci\u00f3n de pge1 genera en los hepatocios frente a dicha muerte celular.  material y m\u00e9todos  los hepatocitos se aislaron de ratas macho tipo wistar mediante el m\u00e9todo cl\u00e1sico de perfusi\u00f3n con colagenasa. Las c\u00e9lulas se cultivaron en medio williams e. Pge1 (1um) se administr\u00f3 2 horas antes que d-galn (0,3, 5, 40mm). La necrosis celular se e valu\u00f3 mediante cuantificaci\u00f3n de actividad lactato deshirogenasas al medio de cultivo. La apoptosis mediante determinaci\u00f3n del grado de framentaci\u00f3n de dna y activaci\u00f3n de caspasas. La producci\u00f3n de \u00f3xido n\u00edtrico (on) se determin\u00f3 por cuantificaci\u00f3n de nitrato+nitrito, mediante el reactivo de griess. Los niveles de mrna y prote\u00edna de \u00f3xido n\u00edtrico sintasa inducible (inos) mediante<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Regulaci\u00f3n de \u00f3xido n\u00edtrico sintasa inducible en la citoprotecci\u00f3n por pge1 de la muerte celular inducida por d-galactosamina en cultivo primario de hepatocitos de rata<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Regulaci\u00f3n de \u00f3xido n\u00edtrico sintasa inducible en la citoprotecci\u00f3n por pge1 de la muerte celular inducida por d-galactosamina en cultivo primario de hepatocitos de rata <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Emilio Siendones Castillo <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 C\u00f3rdoba<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 11\/12\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jordi Muntan\u00e9 Relat<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francisco javier Padillo ruiz <\/li>\n<li>lisardo Bosc\u00e1 gomar (vocal)<\/li>\n<li>pl\u00e1cido Navas lloret (vocal)<\/li>\n<li>Jos\u00e9 Manuel Villalba montoro (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Emilio Siendones Castillo Introducci\u00f3n la administraci\u00f3n de d-galactosamina (d-galn) es un modelo experimental de toxicidad hep\u00e1tica adecuado [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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