{"id":27645,"date":"2018-03-09T09:20:00","date_gmt":"2018-03-09T09:20:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/defectos-en-traduccion-y-transcripcion-por-arn-polimerasa-iii-en-mutantes-gcd16-de-saccharomyces-cerevisiae\/"},"modified":"2018-03-09T09:20:00","modified_gmt":"2018-03-09T09:20:00","slug":"defectos-en-traduccion-y-transcripcion-por-arn-polimerasa-iii-en-mutantes-gcd16-de-saccharomyces-cerevisiae","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/salamanca\/defectos-en-traduccion-y-transcripcion-por-arn-polimerasa-iii-en-mutantes-gcd16-de-saccharomyces-cerevisiae\/","title":{"rendered":"Defectos en traducci\u00f3n y transcripci\u00f3n por arn polimerasa iii en mutantes gcd16 de saccharomyces cerevisiae."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Hernandez Hidalgo M. Estela <\/strong><\/h2>\n<p>Estamos interesados en identificar funciones g\u00e9nicas esenciales para el inicio de la traducci\u00f3n en eucariotas. Nuestra aproximaci\u00f3n consiste en caracterizar mutantes espont\u00e1neos gcd de saccharomyces cerevisiae que tienen alterada la regulaci\u00f3n traduccional de gcn4, clonar los genes correspondientes e investigar su funci\u00f3n en iniciaci\u00f3n y en reiniciaci\u00f3n de la traducci\u00f3n. gcn4 codifica un activador transcripcional de 539 genes que se activan en respuesta a la privaci\u00f3n de amino\u00e1cidos. Su expresi\u00f3n est\u00e1 regulada en funci\u00f3n de la disponibilidad de los mismo, por un mecanismo de reiniciaci\u00f3n de la traducci\u00f3n que depende de cuatro fases cortas de lectura abierta (uorfs) en el l\u00edder arnm-gcn4 y de m\u00faltiples efectores positivos gcnp y negativos gcdp. Las primeras prote\u00ednas gcdp identificadas son subunidades de factores de iniciaci\u00f3n (eifs) y las \u00faltimas, gcd10p y gcd14p, tienen funciones esenciales para el procesamiento del arnt\u00c2\u00a1met. Todas ellas est\u00e1n conservadas en eucariotas superiores. El gen gcd16 codifica rpc34p, una subunidad espec\u00edfica de la arn polimerasa iii; los mutantes correspondientes tiene desreprimida constitutivamente la traducci\u00f3n de gcn4, muestran crecimiento lento incondicional y son termosensibles. Sus perfiles de polisomas revelan defectos en iniciaci\u00f3n de la traducci\u00f3n, concomitantes con un d\u00e9ficit importante de subunidades ribosomales 60s. La mutaci\u00f3n gcd16-1 determina la s\u00edntesis de una prote\u00edna truncada rpc34a21p que es dominante negativa, causando acumulaci\u00f3n de precursores del arnt\u00c2\u00a1met y disminuciones del 50% en la mol\u00e9cula madura que sugieren un defecto en su procesamiento y en transcripci\u00f3n por arn polimerasa iii. Estos y otros datos indican que s\u00f3lo mutaciones que afectan a la arn polimerasa iii reduciendo diferencialmente la biog\u00e9nesis del arnt\u00c2\u00a1met desreprimen la traducci\u00f3n de gcn4 y que \u00e9sta tiene unos requerimientos especiales en relaci\u00f3n a otros arnts.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Defectos en traducci\u00f3n y transcripci\u00f3n por arn polimerasa iii en mutantes gcd16 de saccharomyces cerevisiae.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Defectos en traducci\u00f3n y transcripci\u00f3n por arn polimerasa iii en mutantes gcd16 de saccharomyces cerevisiae. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Hernandez Hidalgo M. Estela <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Salamanca<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 15\/12\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mercedes Tamame Gonzalez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francisco Rey iglesias <\/li>\n<li>M\u00aa rosa Esteban ca\u00f1ibano (vocal)<\/li>\n<li>tah\u00eda Benitez fernandez (vocal)<\/li>\n<li>Francisco Navarro gomez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Hernandez Hidalgo M. Estela Estamos interesados en identificar funciones g\u00e9nicas esenciales para el inicio de la traducci\u00f3n [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[9386],"tags":[79237,9511,79236,25736,9510,3242],"class_list":["post-27645","post","type-post","status-publish","format-standard","hentry","category-salamanca","tag-francisco-navarro-gomez","tag-francisco-rey-iglesias","tag-hernandez-hidalgo-m-estela","tag-mercedes-tamame-gonzalez","tag-ma-rosa-esteban-canibano","tag-tahia-benitez-fernandez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/27645","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=27645"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/27645\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=27645"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=27645"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=27645"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}