{"id":27923,"date":"2018-03-09T09:20:24","date_gmt":"2018-03-09T09:20:24","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-de-la-agrupacion-de-genes-de-biosintesis-de-arginina-y-su-relacion-con-la-produccion-de-acido-clavulanico-en-streptomyces-clavuligerus\/"},"modified":"2018-03-09T09:20:24","modified_gmt":"2018-03-09T09:20:24","slug":"caracterizacion-de-la-agrupacion-de-genes-de-biosintesis-de-arginina-y-su-relacion-con-la-produccion-de-acido-clavulanico-en-streptomyces-clavuligerus","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/leon\/caracterizacion-de-la-agrupacion-de-genes-de-biosintesis-de-arginina-y-su-relacion-con-la-produccion-de-acido-clavulanico-en-streptomyces-clavuligerus\/","title":{"rendered":"Caracterizacion de la agrupacion de genes de biosintesis de arginina y su relacion con la produccion de acido clavulanico en streptomyces clavuligerus"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Fuente Barrientos \u00c1lvaro De La <\/strong><\/h2>\n<p>El \u00e1cido clavul\u00e1nico es un potente inhibidor de beta-lactamasas que se produce industrialmente por streptomyces calvuligerus. Se sintetiza a partir de gliceraldeh\u00eddo 3 fosfato y arginina, amino\u00e1cido que resulta limitante para la producci\u00f3n. Por tanto, el conocimiento de la ruta de bios\u00edntesis de arginina, su regulaci\u00f3n y los flujos metab\u00f3licos en diferentes condiciones de cultivo, as\u00ed como su relaci\u00f3n con la producci\u00f3n de \u00e1cido clavul\u00e1nico, son de gran importancia para entender los mecanismos de producci\u00f3n de \u00e1cido clavul\u00e1nico y para el dise\u00f1o racional de estrategias que permitan la superproducci\u00f3n de dicho compuesto.  en el presente trabajo se ha clonado y secuenciado un fragmento de 2115 nucle\u00f3tidos del genoma de s. Clavuligerus en el que se han localizado los genes de bios\u00edntesis de arginina argj y argb. Estos genes forman parte de la agrupaci\u00f3n de genes de bios\u00edntesis de arginina argcjbdrgh y se expresan en un transcrito policistr\u00f3nico argcjbdr.  debido a la elevada similitud entre los genes argj y el marco de lectura abierto orf6, localizado en la agrupaci\u00f3n de genes de bios\u00edntesis de \u00e1cido clavul\u00e1nico y cuya funci\u00f3n es desconocida, se estudi\u00f3 la funci\u00f3n de ambos genes, demostr\u00e1ndose que se comportaban como ornitina acetil transferasas monofuncionales, capaces de complementar mutantes de e. Coli arge.  estudios m\u00e1s detallados utilizando ambas prote\u00ednas purificadas de forma heter\u00f3loga desde e. Coli mostraron que se autoprocesan en una treonina situada en la posici\u00f3n 179 de argj y 181 de orf6, generando una subunidad alfa de 19 kda, correspondiente a la regi\u00f3n aminoterminal y una subnidad beta de 22 kda, correspondiente a la regi\u00f3n carboxilo terminal de las prote\u00ednas, siendo la forma funcional de las prote\u00ednas el heterotetr\u00e1mero (alfa2beta2).  los estudios ezim\u00e1ticos realizados mostraron que ambas prote\u00ednas presentan un mecanismo de reacci\u00f3n de tipo ping-pong bibi con unos valores de las co<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizacion de la agrupacion de genes de biosintesis de arginina y su relacion con la produccion de acido clavulanico en streptomyces clavuligerus<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizacion de la agrupacion de genes de biosintesis de arginina y su relacion con la produccion de acido clavulanico en streptomyces clavuligerus <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Fuente Barrientos \u00c1lvaro De La <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 19\/12\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Paloma Liras Pad\u00edn<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  Francisco Mart\u00edn mart\u00edn <\/li>\n<li>Jos\u00e9 Luis Barredo fuente (vocal)<\/li>\n<li>antonia Herrero moreno (vocal)<\/li>\n<li> Fuente moreno Juan  Luis de la (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Fuente Barrientos \u00c1lvaro De La El \u00e1cido clavul\u00e1nico es un potente inhibidor de beta-lactamasas que se produce [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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