{"id":27996,"date":"2018-03-09T09:20:30","date_gmt":"2018-03-09T09:20:30","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-genetico-inmunoqua%c2%admico-y-biologico-de-mutantes-rugosos-de-brucella-melitensis\/"},"modified":"2018-03-09T09:20:30","modified_gmt":"2018-03-09T09:20:30","slug":"analisis-genetico-inmunoqua%c2%admico-y-biologico-de-mutantes-rugosos-de-brucella-melitensis","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/navarra\/analisis-genetico-inmunoqua%c2%admico-y-biologico-de-mutantes-rugosos-de-brucella-melitensis\/","title":{"rendered":"An\u00e1lisis gen\u00e9tico, inmunoqu\u00edmico y biol\u00f3gico de mutantes rugosos de brucella melitensis"},"content":{"rendered":"<h2>Tesis doctoral de <strong> David Gonz\u00e1lez Fern\u00e1ndez <\/strong><\/h2>\n<p>Mediante mutag\u00e9nesis con transpos\u00f3n se obtuvieron mutantes rugosos (r) de brucella melitensis 16m y h38. Los an\u00e1lisis electrofor\u00e9ticos y del \u00e1cido 3-deoxi-d-manno-2-octulos\u00f3nico (kdo) y los \u00abimmunoblots\u00bb con anticuerpos monoclonales demostraron la falta de la cadena o (o-ps) en lso lipopolisac\u00e1ridos (lps) de estos mutantes.  la caracterizaci\u00f3n de las regiones mutadas confirm\u00f3 el papel de varios genes wbk (wbka, gmd, per, wzm, weca) en la s\u00edntesis de la cadena o y permiti\u00f3 identificar tres genes nuevos en esta misma regi\u00f3n que codifican, putativamente, una glicosil transferasa (wbke), la primasa que liga un aminoaz\u00facar al bactoprenolpirofosfato (weca) y una epimerasa-deshidrogenasa (wbkd). Adem\u00e1s, se identific\u00f3 un nuevo gen (wbob) en la regi\u00f3n wbo, tambi\u00e9n implicado en la s\u00edntesis de la cadena o. Dos genes m\u00e1s, wa**, manbn\u00facleo, se relacionaron con la s\u00edntesis del n\u00facleo del lps y otro, pgm, con la de precursores. los mutantes en las orf bmeii0682, orf bmeii0053 y orf bmeii1134, presentaron fenotipo r, pero no se les pudo asignar un papel en la s\u00edntesis del lps. los perfiles electrofor\u00e9ticos y el contenido en kdo de los lpss fueron coherentes con los datos gen\u00f3micos y permitieron clasificar los lps-r en tres tipos: r1, con el n\u00facleo del lps aparentemente completo, y r2 y r3 con carencias progresivas en el mismo. El an\u00e1lisis con anticuerpos monoclonales demostr\u00f3 que el n\u00facleo del lps de b.Melitensis es distinto del de b.Abortus.  los mutantes r de b.Melitensis mostraron una superficie m\u00e1s hidr\u00f3foba que las cepas parentales, y tambi\u00e9n que mutantes hom\u00f3logos r de b.Abortus. otras diferencias entre los mutantes r y las cepas parentales fueron su mayor sensibilidad a la penicilina y resistencia a la oxacilina, su marcada carga de superficie, su sensibilidad a la polimixina b y al fago r\/c y la accesibilidad de prote\u00ednas de membrana externa a los anticuerpos.  los mutantes r de b.Melitensis fueron m\u00e1s sensible<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis gen\u00e9tico, inmunoqu\u00edmico y biol\u00f3gico de mutantes rugosos de brucella melitensis<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis gen\u00e9tico, inmunoqu\u00edmico y biol\u00f3gico de mutantes rugosos de brucella melitensis <\/li>\n<li><strong>Autor:<\/strong>\u00a0 David Gonz\u00e1lez Fern\u00e1ndez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 19\/12\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Ignacio Moriyon Uria<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  evaristo Suarez fernandez <\/li>\n<li>Jes\u00fas Ag\u00ed\u00bcero balb\u00edn (vocal)<\/li>\n<li>Jos\u00e9 Mar\u00eda Blasco Martinez (vocal)<\/li>\n<li>marina Garc\u00eda delgado (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de David Gonz\u00e1lez Fern\u00e1ndez Mediante mutag\u00e9nesis con transpos\u00f3n se obtuvieron mutantes rugosos (r) de brucella melitensis 16m y [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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