{"id":28109,"date":"2004-12-01T00:00:00","date_gmt":"2004-12-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/deteccion-clonacion-y-caracterizacion-de-genes-expresados-diferencialmente-en-glucosa-o-lactosa-en-penicillium-chrysogenum\/"},"modified":"2004-12-01T00:00:00","modified_gmt":"2004-12-01T00:00:00","slug":"deteccion-clonacion-y-caracterizacion-de-genes-expresados-diferencialmente-en-glucosa-o-lactosa-en-penicillium-chrysogenum","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/leon\/deteccion-clonacion-y-caracterizacion-de-genes-expresados-diferencialmente-en-glucosa-o-lactosa-en-penicillium-chrysogenum\/","title":{"rendered":"Deteccion, clonacion y caracterizacion de genes expresados diferencialmente en glucosa o lactosa en penicillium chrysogenum"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Castillo Orjuela Nancy Isabel <\/strong><\/h2>\n<p>Las diferentes t\u00e9cnicas para la determinaci\u00f3n de diferencias en la expresi\u00f3n g\u00e9nica entre dos tipos de c\u00e9lulas, o dos condiciones diferentes en que se desarrollan las c\u00e9lulas, constituyen en la actualidad una herramienta de importancia fundamental para dilucidar muchos aspectos importantes de la biolog\u00eda molecular de los organismos, en particular en aspectos relacinados con la regulaci\u00f3n temporal y espacial de diferentes grupos de genes que funcionan de forma coordinada para desarrollar distintas funciones celulares.  con el fin de obtener un conocimiento m\u00e1s amplio del metabolismo primario de penicillium chrysogenum, adem\u00e1s de tratar de encontrar genes que pudieran tener influencia sobre la bios\u00edntesis de penicilina, bien por regular directamente los genes de la ruta o bien por cualquier otro mecanismo indirecto, se clonaron y caracterizaron clones de adnc obtenidos de c\u00e9lulas p. Chrysogenum nrrl 1951 (cepa silvestre) crecidas en medios con glucosa (fuente represora de la bios\u00edntesis de penicilina) o lactosa (fuente no represora) como fuentes de carbono. Para ello se utiliz\u00f3 la t\u00e9cnica de substracci\u00f3n de adn y selecci\u00f3n por pcr (kit pcr-select tm cdna substraction de la casa comercial clontech).  de los clones caracterizados, 55 presentaron identidad con diferentes prote\u00ednas conocidas en las bases de datos utilizadas, mientras que 92 no presentaron identidad significativa con ninguna prote\u00edna ya caracterizada. El an\u00e1lisis de los clones condujo a la caracterizaci\u00f3n de varios genes no identificados a\u00fan en p. Chrysogenum.  algunos de los clones obtenidos y que presentan una expresi\u00f3n diferencial dr\u00e1stica est\u00e1n involucrados en la respiraci\u00f3n. El an\u00e1lisis y caracterizaci\u00f3n completos de estos genes podr\u00eda contribuir a elucidar mecanismos regulares o rutas de transducci\u00f3n de se\u00f1ales involucradas en la generaci\u00f3n de energ\u00eda en p. Chrysogenum.  en resumen, en este trabajo se analiz\u00f3 una peque\u00f1a parte del genom<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Deteccion, clonacion y caracterizacion de genes expresados diferencialmente en glucosa o lactosa en penicillium chrysogenum<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Deteccion, clonacion y caracterizacion de genes expresados diferencialmente en glucosa o lactosa en penicillium chrysogenum <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Castillo Orjuela Nancy Isabel <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 12\/01\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Francisco Mart\u00edn Mart\u00edn<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Antonio  gerardo Pisabarro de lucas <\/li>\n<li>Jes\u00fas Manuel Cantoral fern\u00e1ndez (vocal)<\/li>\n<li>lucia Ramirez nasto (vocal)<\/li>\n<li>bruno D\u00edez Garc\u00eda (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Castillo Orjuela Nancy Isabel Las diferentes t\u00e9cnicas para la determinaci\u00f3n de diferencias en la expresi\u00f3n g\u00e9nica entre [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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