{"id":28430,"date":"2018-03-09T09:21:07","date_gmt":"2018-03-09T09:21:07","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-molecular-de-la-represion-por-catabolito-e-ingenieria-metabolica-en-lactobacillus-casei\/"},"modified":"2018-03-09T09:21:07","modified_gmt":"2018-03-09T09:21:07","slug":"caracterizacion-molecular-de-la-represion-por-catabolito-e-ingenieria-metabolica-en-lactobacillus-casei","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-molecular-de-la-represion-por-catabolito-e-ingenieria-metabolica-en-lactobacillus-casei\/","title":{"rendered":"Caracterizaci\u00f3n molecular de la represi\u00f3n por catabolito e ingenier\u00eda metab\u00f3lica en lactobacillus casei"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Esteban Nieto Carlos David <\/strong><\/h2>\n<p>En bacterias l\u00e1cticas, los genes del metabolismo de carbohidratos normalmente presentan un doble sistema de regulaci\u00f3n para su expresi\u00f3n: represi\u00f3n por catabolito, mediada por la prote\u00edna ccpa e inducci\u00f3n por sustrato. En el oper\u00f3n lac de lactobacillus casei la responsable de la inducci\u00f3n por sustrato es la prote\u00edna antiterminadora lact. En la presente tesis se han estudiado estos dos fen\u00f3menos.  se ha realizado una an\u00e1lisis mutacional de los dominios de uni\u00f3n al correpresor y al dna de la prote\u00edna ccpa. Se han identificado dos mutaciones en el dominio de uni\u00f3n al dna (t7s y n52s) que confieren desrepresi\u00f3n y otras dos, en este caso en el dominio de uni\u00f3n al correpresor (s80l y t307i), que producen un fenotipo de hiperrepresi\u00f3n. La comparaci\u00f3n de estos efectos con los que producen mutaciones equivalentes en bacillus megaterium y un estudio de complementaci\u00f3n realizado en esta misma tesis, indican que aunque en ambas bacterias el proceso de represi\u00f3n por catabolito es funcional, los residuos de amino\u00e1cidos implicados y los cambios moleculares en ccpa causados por la interacci\u00f3n con el cofactor pueden ser diferentes en ambas especies.  se ha estudiado la regulaci\u00f3n de la actividad el antiterminador lact por fosforilaci\u00f3n de las histidinas conservadas de los dominios prd. Se han mutado esta histidinas por asp\u00e1rtico o alanina para mimetizar el estado de fosforilaci\u00f3n o no fosforilaci\u00f3n respectivamente. Los resultados obtenidos indican que las histidinas del prd-i deben estar desfosforiladas para desempe\u00f1ar la inducci\u00f3n por lactosa y que las histidina del prd-ii est\u00e1n implicadas en la represi\u00f3n por glucosa del oper\u00f3n lac en un proceso independiente de ccpa.  por \u00faltimo, se desarrollo un sistema de integraci\u00f3n de genes dentro del oper\u00f3n lac para obtener su expresi\u00f3n regulada por ccpa y lact. Se ha comprobado el buen funcionamiento  de este vector y se ha empleado para expresar el enzima acetohidroxi\u00e1cido<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n molecular de la represi\u00f3n por catabolito e ingenier\u00eda metab\u00f3lica en lactobacillus casei<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n molecular de la represi\u00f3n por catabolito e ingenier\u00eda metab\u00f3lica en lactobacillus casei <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Esteban Nieto Carlos David <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Universitat de val\u00e9ncia (estudi general)<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 30\/01\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Gaspar P\u00e9rez Mart\u00ednez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: daniel Ram\u00f3n vidal <\/li>\n<li>Jos\u00e9 Luis Garcia lopez (vocal)<\/li>\n<li>josef Deustcher (vocal)<\/li>\n<li>Manuel Z\u00fa\u00f1iga cabrera (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Esteban Nieto Carlos David En bacterias l\u00e1cticas, los genes del metabolismo de carbohidratos normalmente presentan un doble [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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