{"id":28650,"date":"2018-03-09T09:21:26","date_gmt":"2018-03-09T09:21:26","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-de-una-peroxirredoxina-cloroplasta%c2%addica-de-pisum-sativum\/"},"modified":"2018-03-09T09:21:26","modified_gmt":"2018-03-09T09:21:26","slug":"caracterizacion-de-una-peroxirredoxina-cloroplasta%c2%addica-de-pisum-sativum","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-de-una-peroxirredoxina-cloroplasta%c2%addica-de-pisum-sativum\/","title":{"rendered":"Caracterizaci\u00f3n de una peroxirredoxina cloroplast\u00eddica de pisum sativum"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Laura Bernier Villamor <\/strong><\/h2>\n<p>En los \u00faltimos a\u00f1os, y en todos los reinos, se ha descrito un nuevo tipo de enzimas, las peroxirredoxinas (prx), capaces de intervenir en los sistemas de destoxificaci\u00f3n de las especies de ox\u00edgeno reactivo reduciendo el h2o2 y distintos alquilhidroper\u00f3xidos. En vegetales superiores una de las enzimas pertenecientes a esta familia, la peroxirredoxina de dos ciste\u00ednas (prx de 2-cys), se localiza en el cloroplasto donde parece estar involucrada en procesos de gran importancia como la protecci\u00f3n  de la fotos\u00edntesis, la destoxificaci\u00f3n de h2o2 a nivel del ciclo agua-agua, la protecci\u00f3n de los tilacoides y tambi\u00e9n en procesos de se\u00f1alizaci\u00f3n celular.  en el estudio presentado en esta tesis doctoral se ha aislado el cdna que codifica la prx de 2-cys de pisum sativum mediante pcr. El cdna aislado codifica una enzima con gran similitud a prx de 2-cys descritas en otros organismos presentando las dos ciste\u00ednas caracter\u00edsticas de este tipo de enzimas as\u00ed como los motivos pept\u00eddicos conservados alrededor de ellas, igualmente presenta otros amino\u00e1cidos esenciales para la actividad de estas enzimas. Se ha sobreexpresado la prote\u00edan recombinante en e.Coli utilizando el sistema de expresi\u00f3n pet. La prote\u00edna recombinante se purific\u00f3 y se llev\u00f3 a cabo una caracterizaci\u00f3n cin\u00e9tica exhaustiva, as\u00ed como la determinaci\u00f3n de ciertas caracter\u00edsticas f\u00edsico-qu\u00edmicas.  se determin\u00f3 que la enzima prsenta dos conformaciones distintas, dim\u00e9rica y decam\u00e9rica, favoreci\u00e9ndose esta segunda por la fuerza i\u00f3nica del medio, el estado redox de la enzima, el ph bajo y el ani\u00f3n fosfato. Se ha demostrado la actividad antioxidante de la enzima presentando propiedades comparables a las descritas para otras enzimas similares y se ha visto que se reduce por tiorredoxina (trx) y ciclofilina. Mediante inmunocitoqu\u00edmica se ha confirmado la localizaci\u00f3n cloroplast\u00eddica de la prx de 2-cys de p.Sativum, dentro del cloroplasto la enzima se localiza f<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n de una peroxirredoxina cloroplast\u00eddica de pisum sativum<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n de una peroxirredoxina cloroplast\u00eddica de pisum sativum <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Laura Bernier Villamor <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Granada<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/02\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> L\u00e1zaro Paniagua Juan  Jos\u00e9<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Carlos G\u00f3mez-moreno calera <\/li>\n<li>Jos\u00e9 Antonio Barcena ruiz (vocal)<\/li>\n<li>Luis alfonso Del rio legazpi (vocal)<\/li>\n<li>francisca Sevilla valenzuela (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Laura Bernier Villamor En los \u00faltimos a\u00f1os, y en todos los reinos, se ha descrito un nuevo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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