{"id":28698,"date":"2018-03-09T09:21:30","date_gmt":"2018-03-09T09:21:30","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/modelos-tridimensionales-de-la-ribonucleoprotea%c2%adna-y-la-polimerasa-recombinantes-del-virus-de-la-gripe\/"},"modified":"2018-03-09T09:21:30","modified_gmt":"2018-03-09T09:21:30","slug":"modelos-tridimensionales-de-la-ribonucleoprotea%c2%adna-y-la-polimerasa-recombinantes-del-virus-de-la-gripe","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/modelos-tridimensionales-de-la-ribonucleoprotea%c2%adna-y-la-polimerasa-recombinantes-del-virus-de-la-gripe\/","title":{"rendered":"Modelos tridimensionales de la ribonucleoprote\u00edna y la polimerasa recombinantes del virus de la gripe"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Estela Area Gomez <\/strong><\/h2>\n<p>El genoma del virus de la gripe est\u00e1 estructurado en forma de ribonucleoprote\u00ednas o rnps, las cuales a su vez  est\u00e1n constituidas por el rna viral, de cadena sencilla y polaridad negativa, cuyos extremos parcialmente complementarios forman una estructura denominada en \u00abmango de sart\u00e9n\u00bb o panhandle.  a lo largo de toda la longitud del rna se sit\u00faa la nucleoprote\u00edna y en su extremo las 3 subunidades de la polimerasa pb1, pb2 y pa, cerrando la estructura de la rnp.  se ha obtenido la estructura tridimensional de una ribonucleoprote\u00edna del virus de la gripe reconstituida in vivo a partir de prote\u00ednas recombinantes y un vrna modelo. Esta estructura, derivada por microscop\u00eda electr\u00f3nica de muestras te\u00f1idas es circular y contiene 9 mon\u00f3meros de nucleoprote\u00edna y el complejo de la polimerasa conectado a dos de estas nucleoprote\u00ednas adyacentes.  se ha optimizado el proceso de purificaci\u00f3n de ribonucleoprote\u00ednas mediante la introducci\u00f3n de un tag de histidinas en una de las prote\u00ednas recombinantes expresadas in vivo. El complejo as\u00ed reconstitu\u00eddo fue purificado mediante cromatograf\u00eda de afinidad a resina de n\u00edquel. La estructura de la polimerasa viral presente en el complejo de la ribonucleoprote\u00edna fue determinada mediante reconstrucci\u00f3n tridimensional de muestras de im\u00e1genes de microscop\u00eda electr\u00f3nica de muestras te\u00f1idas. Con una resoluci\u00f3n de 25a, la estructura obtenida muetra la presencia de sus tres subunidades. La localizaci\u00f3n de dominios espec\u00edficos en la polimerasa fue determinada mediante an\u00e1lisis tridimensional de complejos de ribonucleoprote\u00ednas unidas a anticuerpos monoclonales espec\u00edficos para el dominio aminoterminal de pb2 y el dominio carboxiterminal de pa. La presencia de masas extra en el complejo, que corresponden a los anticuerpos unidos, indica la posici\u00f3n de dichos dominios de las subunidades pb2 y pa dentro de la polimerasa. La localizaci\u00f3n de dominios espec\u00edficos de la subunidad pb1 se l<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Modelos tridimensionales de la ribonucleoprote\u00edna y la polimerasa recombinantes del virus de la gripe<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Modelos tridimensionales de la ribonucleoprote\u00edna y la polimerasa recombinantes del virus de la gripe <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Estela Area Gomez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 16\/02\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan Ortin Monton<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francisco Parra fernandez <\/li>\n<li>crisanto Gutierrez armenta (vocal)<\/li>\n<li> Rodr\u00edguez aguirre Jos\u00e9 Francisco (vocal)<\/li>\n<li>Jos\u00e9 Mar\u00eda Carazo garcia (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Estela Area Gomez El genoma del virus de la gripe est\u00e1 estructurado en forma de ribonucleoprote\u00ednas o [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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