{"id":28916,"date":"2018-03-09T09:21:49","date_gmt":"2018-03-09T09:21:49","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-del-sistema-regulador-de-dos-componentes-bvrr-bvrs-de-brucella-abortus\/"},"modified":"2018-03-09T09:21:49","modified_gmt":"2018-03-09T09:21:49","slug":"caracterizacion-del-sistema-regulador-de-dos-componentes-bvrr-bvrs-de-brucella-abortus","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/navarra\/caracterizacion-del-sistema-regulador-de-dos-componentes-bvrr-bvrs-de-brucella-abortus\/","title":{"rendered":"Caracterizaci\u00f3n del sistema regulador de dos componentes bvrr\/bvrs de brucella abortus"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Lorea Manterola Kareaga <\/strong><\/h2>\n<p>El an\u00e1lisis mediante 2-dge revel\u00f3 que los mutantes en el sistema bvrr\/bvrs no expresaban ciertas prote\u00ednas de membrana externa (omp) y expresaban otras no presentes en la cepa parental. Mediante western-blot con anticuerpos monoclonales se identific\u00f3 que una de las prote\u00ednas ausentes en los mutantes era la omp25. La microsecuenciaci\u00f3n de las prote\u00ednas extra\u00eddas de los geles de 2-dge mediante maldi-ms permiti\u00f3 identificar otra de las omps no descrita hasta la fecha, la omp22. El control de la expresi\u00f3n de la omp25 y omp22 por parte del sistema regulador de dos componentes ten\u00eda lugar a nivel transcripcional, ya que la actividad de los promotores p omp25 y p omp22 estaba reducida en los mutantes bvrr\/bvrs. Se identific\u00f3 el estr\u00e9s nutricional como factor que modula la expresi\u00f3n de ambas omps. No fue posible identificar las omps cuya expresi\u00f3n parec\u00eda estar reprimida por el sistema bvrr\/bvrs en condiciones de cultivo de laboratorio. A diferencia de lo que ocurr\u00eda con los mutantes bvrr\/bvrs, la mutaci\u00f3n en las prote\u00ednas omp25 y omp22 no afect\u00f3 a la sensibilidad a la polimixina b, a la sensibilidad a la acci\u00f3n bactericida del suero normal de vaca y humano, a la virulencia en el modelo de rat\u00f3n, a la multiplicaci\u00f3n intracelular en macr\u00f3fagos y c\u00e9lulas hela, ni a la internalizaci\u00f3n en las c\u00e9lulas hela. Estos resultados indicaban que adem\u00e1s de controlar la expresi\u00f3n de la omp25 y omp22, el sistema bvrr\/bvrs deb\u00eda regular otros factores implicados en la virulencia de brucella, por lo que se analiz\u00f3 la composici\u00f3n del lps.  las t\u00e9cnicas empleadas, sds-page e inmunoblot con anticuerpos espec\u00edficos, no permitieron detectar alteraciones en el n\u00facleo de un doble mutante rugosos bvrr::tn5 aper. Sin embargo, se detect\u00f3 un mayor grado de acilaci\u00f3n del l\u00edpido a y una mayor fluidez de las cadenas acilo en el lps de los mutantes bvrr\/bvrs. Por otro lado, el mutante bvrr pero no el bvrs present\u00f3 problemas de crecimiento en<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n del sistema regulador de dos componentes bvrr\/bvrs de brucella abortus<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n del sistema regulador de dos componentes bvrr\/bvrs de brucella abortus <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Lorea Manterola Kareaga <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 27\/02\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Ignacio Lopez Go\u00f1i<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ignacio Moriyon uria <\/li>\n<li>Francisco Garcia del portillo (vocal)<\/li>\n<li>Juan SanJuan (vocal)<\/li>\n<li>i\u00f1igo Lasa (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Lorea Manterola Kareaga El an\u00e1lisis mediante 2-dge revel\u00f3 que los mutantes en el sistema bvrr\/bvrs no expresaban [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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