{"id":29255,"date":"2018-03-09T09:22:18","date_gmt":"2018-03-09T09:22:18","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-de-los-cambios-de-expresion-genica-relacionados-con-la-invasion-tumoral-y-la-metastasis-en-adenocarcinoma-mamario-murino\/"},"modified":"2018-03-09T09:22:18","modified_gmt":"2018-03-09T09:22:18","slug":"estudio-de-los-cambios-de-expresion-genica-relacionados-con-la-invasion-tumoral-y-la-metastasis-en-adenocarcinoma-mamario-murino","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-de-los-cambios-de-expresion-genica-relacionados-con-la-invasion-tumoral-y-la-metastasis-en-adenocarcinoma-mamario-murino\/","title":{"rendered":"Estudio de los cambios de expresi\u00f3n g\u00e9nica relacionados con la invasi\u00f3n tumoral y la met\u00e1stasis en adenocarcinoma mamario murino"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Marc Adrover Blanch <\/strong><\/h2>\n<p>Con la finalidad de identificar genes involucrados en la met\u00e1stasis , hemos utilizado un modelo de c\u00e1ncer de mama murino. Se han aislado una serie de clones con diferentes caracter\u00edsticas celulares, capacidad metast\u00e1sica y dependencia hormonal, pero gen\u00e9ticamente relacionados al tener origen com\u00fan en la l\u00ednea celular parental m xt s. Para demostrar el fondo gen\u00f3mico com\u00fan y la clonalidad de las variantes del modelo mxt hemos utilizado el estudio por citometr\u00eda, citogen\u00e9tica y dna fingerprinting.  para investigar los genes diferencialmente expresados por los clones derivados de mxt, hemos utilizado la t\u00e9cnica del rna arbitraly primed (rap-pcr). se obtuvieron rna fingerprints de cada uno de los clones, los cuales se compararon y se encontraron diferencias en bandas espec\u00edficas. Tras la selecci\u00f3n y clonaje de estas bandas, se aislaron cdna que se utilizaron como sondas para verificar las diferencias entre los niveles de expresi\u00f3n entre los distintos clones mediante northern blot. El an\u00e1lisis de la secuencia de una de las seleccionada se identific\u00f3 coom sparc (secreted protein acidic and rich in cystein), una glicopote\u00edna que ha sido relacionada con las interacciones entre c\u00e9lulas y matriz extracelular durante la remodelaci\u00f3n, morfog\u00e9nesis, migraci\u00f3n y proliferaci\u00f3n. Este gen se encontr\u00f3 sobrexpresado en las l\u00edneas celulares m\u00e1s metast\u00e1sicas mxt c1.1 y mxt b2 en relaci\u00f3n a la l\u00ednea parenteal m xt s, la cual muestra elevada capacidad tumorog\u00e9nica, pero baja capacidad metast\u00e1sica.  con el objetivo de modificar la expresi\u00f3n g\u00e9nica de los genes sparc y de la metaloproteinasa 2 (mmp2) de las c\u00e9lulas del modelo mxt, y concretamente las l\u00edneas mxt b2 y mxt e2, hemos utilizado vectores de expresi\u00f3n. Se ha modificado la expresi\u00f3n de lso genes sparc y  mmp2 de c\u00e9lulas de la l\u00ednea mxt b2.  los clones obtenidos presentaban la expresi\u00f3n del gen sparc reducida, la expresi\u00f3n alta de mmp2 y otros clones expresaban n<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio de los cambios de expresi\u00f3n g\u00e9nica relacionados con la invasi\u00f3n tumoral y la met\u00e1stasis en adenocarcinoma mamario murino<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio de los cambios de expresi\u00f3n g\u00e9nica relacionados con la invasi\u00f3n tumoral y la met\u00e1stasis en adenocarcinoma mamario murino <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Marc Adrover Blanch <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 18\/03\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Angels Fabra Fres<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: amparo Cano garcia <\/li>\n<li>jaume Piulats xanc\u00f3 (vocal)<\/li>\n<li>anna Mar\u00eda Bassols teixido (vocal)<\/li>\n<li>Miguel Quintanilla (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Marc Adrover Blanch Con la finalidad de identificar genes involucrados en la met\u00e1stasis , hemos utilizado un [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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