{"id":29259,"date":"2018-03-09T09:22:18","date_gmt":"2018-03-09T09:22:18","guid":{"rendered":""},"modified":"2018-03-09T09:22:18","modified_gmt":"2018-03-09T09:22:18","slug":"identificacion-de-daxx-y-kis-como-protea%c2%adnas-que-interaccionan-con-la-senal-de-retencion-dileucina-contenida-en-el-dominio-carboxilo-terminal-de-glut4-el-transportador-de-glucosa-sensible-a-insu","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-de-daxx-y-kis-como-protea%c2%adnas-que-interaccionan-con-la-senal-de-retencion-dileucina-contenida-en-el-dominio-carboxilo-terminal-de-glut4-el-transportador-de-glucosa-sensible-a-insu\/","title":{"rendered":"Identificaci\u00f3n de daxx y kis como prote\u00ednas que interaccionan con la se\u00f1al de retenci\u00f3n dileucina contenida en el dominio carboxilo terminal de glut4, el transportador de glucosa sensible a insulina"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Silvia Vergara Jauregui Letamendia <\/strong><\/h2>\n<p>En una b\u00fasqueda de prote\u00ednas que interaccionaban f\u00edsicamente con el dominio c-terminal de glut4, con el sistema de doble h\u00edbrido de levadura, identificamos el dominio carboxilo de daxx y el dominio carboxilo terminal de una prote\u00edna desconocida a la que denominamos kis. La intervenci\u00f3n encontrada por el doble h\u00edbrido entre c-glut4 y c-kis y c-glut4 y c-daxx fue validada por la capacidad del c-glut4 traducido in vitro de interaccionar con el daxx silvestre, el c-daxx y el c-kis traducido in vitro. C-daxx y c-kis no interaccionan con el dominio c-citoplasm\u00e1tico de glut1, el transportador de glucosa expresado de forma ubicua y hom\u00f3logo a glut4. La anulaci\u00f3n de la se\u00f1al dileucina (leu489leu790), cr\u00edtica para el transporte de glut4 desde la red trans del golgi al compartimento perinuclear de almacenaje, y para su internalizaci\u00f3n desde la superficie celular por endocitosis, por la substituci\u00f3n del par leuleu por el par ala-ser inhibi\u00f3 en un 50% la interacci\u00f3n entre c-glut4 y c-daxx, mientras que la introducci\u00f3n de una alanina entre las dos arg que preceden al par dileucina, inhibi\u00f3 completamente la interacci\u00f3n de c-kis y c-glut4.  la daxx y el glut4 contenidos en fibroblastos 3t3-l1 transfectados establemente con glut4, as\u00ed como en adipocitos 3t3-l1, son coinmunoprecipitados con anticuerpos contra glut4 y daxx, respectivamente. Utilizando un procedimiento bien establecido de fraccionamiento celular, demostramos evidencia de la existencia de dos poblaciones intracelulares de daxx en el n\u00facleo y en los microsomas de baja densidad. Estudios de microscop\u00eda confocal localizaron a daxx en los cuerpos pods y en estructuras vesiculares en el citoplasma, frecuentemente organizadas en hileras y bajo la membrana plasm\u00e1tica. Tambi\u00e9n presentamos evidencias de que tanto glut4 como daxx son conjugadas a sumo-1.  hemos caracterizado una nueva prote\u00edna a la que hemos denominado kis, codificada por un mrna de 2.4kb. Sorprendentemente, el<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Identificaci\u00f3n de daxx y kis como prote\u00ednas que interaccionan con la se\u00f1al de retenci\u00f3n dileucina contenida en el dominio carboxilo terminal de glut4, el transportador de glucosa sensible a insulina<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Identificaci\u00f3n de daxx y kis como prote\u00ednas que interaccionan con la se\u00f1al de retenci\u00f3n dileucina contenida en el dominio carboxilo terminal de glut4, el transportador de glucosa sensible a insulina <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Silvia Vergara Jauregui Letamendia <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 18\/03\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Vassiliki Lalioti<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: balbino jose Alarcon sanchez <\/li>\n<li>dionisio Miguel Mart\u00edn zanca (vocal)<\/li>\n<li>isabel Guerrero vega (vocal)<\/li>\n<li>Jos\u00e9 Gonz\u00e1lez casta\u00f1o (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Silvia Vergara Jauregui Letamendia En una b\u00fasqueda de prote\u00ednas que interaccionaban f\u00edsicamente con el dominio c-terminal de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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