{"id":29440,"date":"2018-03-09T09:22:34","date_gmt":"2018-03-09T09:22:34","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/disseny-sa%c2%adntesi-i-estudi-de-lligands-pepta%c2%addics-capaa%c2%a7os-de-recona%c2%a8ixer-la-superfa%c2%adcie-de-la-p53\/"},"modified":"2018-03-09T09:22:34","modified_gmt":"2018-03-09T09:22:34","slug":"disseny-sa%c2%adntesi-i-estudi-de-lligands-pepta%c2%addics-capaa%c2%a7os-de-recona%c2%a8ixer-la-superfa%c2%adcie-de-la-p53","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/polipeptidos-y-proteinas\/disseny-sa%c2%adntesi-i-estudi-de-lligands-pepta%c2%addics-capaa%c2%a7os-de-recona%c2%a8ixer-la-superfa%c2%adcie-de-la-p53\/","title":{"rendered":"Disseny, s\u00edntesi i estudi de lligands pept\u00eddics capa\u00c1\u00a7os de recon\u00e9ixer la superf\u00edcie de la p53."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Marc Martinel Pedemonte <\/strong><\/h2>\n<p>Dada la importancia del reconocimiento de superficies proteicas, este trabajo de tesis doctoral se ha dirigido a la utilizaci\u00f3n de ligandos pept\u00eddicos para el reconocimiento de superficies proteicas ricas en carboxilatos. en concreto, como diana, se ha escogido el dominio de tetramerizaci\u00f3n de la prote\u00edna supresora de tumores p53.  la p53 es una importante diana en la investigaci\u00f3n contra el c\u00e1ncer dado su papel clave en el control de la integridad del adn. As\u00ed pues, los ligandos que puedan modular su funci\u00f3n, poseen un importante potencial como agentes terap\u00e9uticos.  en una primera etapa, se ha puesto a punto la metodolog\u00eda necesaria para poder obtener, mediante expresi\u00f3n en cultivos bacterianos, el dominio de  tetramerizaci\u00f3n de la p53 con o sin marca isot\u00f3pica de 13c y\/o  15n.  posteriormente, mediante herramientas de modelado molecular se ha dise\u00f1ado un p\u00e9ptido (ac-agaagwargrarsr-nh2) el cual es capaz de reconocer la superficie rica en carboxilatos de la p53.  la interacci\u00f3n se ha estudiado mediante resonancia magn\u00e9tica nuclear, resonancia de plasm\u00f3n superficial (biacore), espectroscopia de fluoresencia y microcalorimetria, encontr\u00e1ndose una constante de afinidad del orden de 10 micromolar. Adem\u00e1s, se ha comprobado que el p\u00e9ptido es capaz de cruzar la membrana celular.  a partir de este p\u00e9ptido, se ha dise\u00f1ado y preparado una quimioteca de 40 p\u00e9ptidos que ha permitido entender cuales son los factores que gobiernan este proceso de reconocimiento, y ha pretendido obtener 4 p\u00e9ptidos con una afinidad hasta un orden de magnitud superior.  finalmente, se han obtenido anticuerpos dirigidos a dos regiones distintas del dominio de tetramerizaci\u00f3n de la p53.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Disseny, s\u00edntesi i estudi de lligands pept\u00eddics capa\u00c1\u00a7os de recon\u00e9ixer la superf\u00edcie de la p53.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Disseny, s\u00edntesi i estudi de lligands pept\u00eddics capa\u00c1\u00a7os de recon\u00e9ixer la superf\u00edcie de la p53. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Marc Martinel Pedemonte <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 29\/03\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Ernest Giralt Lledo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: fernando Albericio palomera <\/li>\n<li>david Andreu mart\u00ednez (vocal)<\/li>\n<li>Jos\u00e9 Fuentes mota (vocal)<\/li>\n<li>enrique Perez paya (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Marc Martinel Pedemonte Dada la importancia del reconocimiento de superficies proteicas, este trabajo de tesis doctoral se 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