{"id":29682,"date":"2018-03-09T09:22:55","date_gmt":"2018-03-09T09:22:55","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-caracterizacion-y-purificacion-de-protea%c2%adnas-capaces-de-ligar-acidos-grasos-en-trypanosoma-cruzi\/"},"modified":"2018-03-09T09:22:55","modified_gmt":"2018-03-09T09:22:55","slug":"aislamiento-caracterizacion-y-purificacion-de-protea%c2%adnas-capaces-de-ligar-acidos-grasos-en-trypanosoma-cruzi","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-caracterizacion-y-purificacion-de-protea%c2%adnas-capaces-de-ligar-acidos-grasos-en-trypanosoma-cruzi\/","title":{"rendered":"Aislamiento, caracterizaci\u00f3n y purificaci\u00f3n de prote\u00ednas capaces de ligar \u00e1cidos grasos en trypanosoma cruzi"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  C\u00f3rdova Paz Sold\u00e1n Ofelia Magdalena <\/strong><\/h2>\n<p>Trypanosoma cruzi, agente etiol\u00f3gico de la enfermedad de chagas, es un protozoo que como la mayor parte de los protozoos par\u00e1sitos, se caracteriza por una poseer una estrecha dependencia metab\u00f3lica del hospedador. La imposibilidad de sintetizar sus propios \u00e1cidos grasos tanto para su metabolismo energ\u00e9tico, como para la s\u00edntesis de nuevas membranas, supone la necesidad de captar dichos \u00e1cidos grasos bien por un proceso de difusi\u00f3n a trav\u00e9s de las membranas o mediante la presencia de transportadores espec\u00edficos de este proceso, como las prote\u00ednas capaces de ligar los \u00e1cidos grasos fatty acid binding protein \u00abfabp\u00bb , descritas en otros organismos.  la existencia de prote\u00ednas capaces de ligar \u00e1cidos grasos ha sido descrita en helmitos par\u00e1sitos como schistosoma mansoni \u00absm14-fabp\u00bb, schistosoma japonicum, fasciola hepatica \u00abfh15-fabp\u00bb, fasciola gigantica \u00abfg-fabp\u00bb, echinococcus granulosus, moniezia expansa, ascaris suum as-p18, bbrujia malayi \u00abbm-fab-1\u00bb, en algunos protozoos par\u00e1sitos incluso m\u00e1s primitivos evolutivamente hablando como giardia y leishmania.  empleando t\u00e9cnicas cromatogr\u00e1ficas se logr\u00f3 purificar estas prote\u00ednas capaces de ligar \u00e1cidos grasos de t.Cruzi, mostrando ser una prote\u00edna oligomerica de aproximadamente de 60 kda en su estado nativo, que mediante t\u00e9cnicas electrofor\u00e9ticas en sds-page, da monomeros entre 45 a 10 kda, sendo la de 10 kda la de mayor inter\u00e9s, por su propiedad de ligar \u00e1cidos grasos. los estudios de isoelectroenfoque de esta prote\u00edna, revela que posee un punto isoel\u00e9ctrico (pi) de 3,39, similar al observado en protoxxos pat\u00f3genos como giardia y leishmania, indicando un car\u00e1cter \u00e1cido de esta prote\u00edna.  la determinaci\u00f3n de la caracter\u00edstica de equilibrio de la prote\u00edna purificada de t.Cruzi al \u00e1cido oleico marcado radiactivamente 14c y los resultados sometidos a an\u00e1lisis de scatchard, muestran un sitio con alta selectividad (kd de 1.15 um+-0.19) y un sitio de uni\u00f3n de 0<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aislamiento, caracterizaci\u00f3n y purificaci\u00f3n de prote\u00ednas capaces de ligar \u00e1cidos grasos en trypanosoma cruzi<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aislamiento, caracterizaci\u00f3n y purificaci\u00f3n de prote\u00ednas capaces de ligar \u00e1cidos grasos en trypanosoma cruzi <\/li>\n<li><strong>Autor:<\/strong>\u00a0  C\u00f3rdova Paz Sold\u00e1n Ofelia Magdalena <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Granada<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 19\/04\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Antonio Osuna Carrillo De Albornoz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: v Hillyer george <\/li>\n<li>Antonio Mart\u00ednez fern\u00e1ndez (vocal)<\/li>\n<li> Espino hern\u00e1ndez Ana margarita (vocal)<\/li>\n<li>Rafael Jim\u00e9nez medina (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de C\u00f3rdova Paz Sold\u00e1n Ofelia Magdalena Trypanosoma cruzi, agente etiol\u00f3gico de la enfermedad de chagas, es un protozoo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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