{"id":29759,"date":"2018-03-09T09:23:01","date_gmt":"2018-03-09T09:23:01","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/citotoxicidad-inducida-por-cisplatino-estudio-experimental-in-vitro-en-celulas-de-feocromocitoma-de-rata-pc-12\/"},"modified":"2018-03-09T09:23:01","modified_gmt":"2018-03-09T09:23:01","slug":"citotoxicidad-inducida-por-cisplatino-estudio-experimental-in-vitro-en-celulas-de-feocromocitoma-de-rata-pc-12","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/citotoxicidad-inducida-por-cisplatino-estudio-experimental-in-vitro-en-celulas-de-feocromocitoma-de-rata-pc-12\/","title":{"rendered":"Citotoxicidad inducida por cisplatino. estudio experimental in vitro en c\u00e9lulas de feocromocitoma de rata pc-12"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Ramos Navas-parejo Juan  M. <\/strong><\/h2>\n<p>El cisplatino es un agente quimioter\u00e1pico usado ampliamente para el tratamiento de diversos tipos de tumores pero cuya dosis total est\u00e1 limitada por sus efectos adversos, principalmente en ri\u00f1\u00f3n y sistema nervioso perif\u00e9rico. la incidencia de nefropat\u00eda se ha reducido significativamente por las diuresis por cloruros, pero no la neurotoxicidad que es, actualmente, el principal factor limitante de la dosis, lo que disminuye la eficacia anticancerosa de este f\u00e1rmaco.  con la finalidad de profundizar en el conocimiento de los mecanismos involucrados en la citotoxicidad inducida por cisplatino nos planteamos los siguientes objetivos:  1,- establecer un modelo experimental in vitro de citotoxicidad inducida por dosis terap\u00e9uticas de cisplatino mediante la utilizaci\u00f3n de la l\u00ednea celular de feocromocitoma de rata pc12 indiferenciadas.  2,- evaluar las ateraciones en la proliferaci\u00f3n y viabilidad celular provocadas por el cisplatino, mediante el empleo de la sal de tetrazolio vst-1 y azul trip\u00e1n, respectivamente.  3,- evaluar los mecanismos de muerte celular inducida por cisplatino mediante la utilizaci\u00f3n de par\u00e1metros morfol\u00f3gicos (micoscop\u00eda de fluorescencia y electr\u00f3nica de transmisi\u00f3n) y bioqu\u00edmicos (estudio de la fragmentaci\u00f3n del adn).  4,- evaluar las alteraciones en la composici\u00f3n elemental de las c\u00e9lulas pc-12 durante la progresi\u00f3n de la lesi\u00f3n celular inducida por el cisplatino mediante la utilizaci\u00f3n del microan\u00e1lisis por energ\u00eda dispersiva de rayos x.  nuestros resultados demuestran que el cisplatino inhibe la proliferaci\u00f3n de las c\u00e9lulas pc-12 debido a la inducci\u00f3n de la muerte de dichas c\u00e9lulas. \u00e9stas muestran con microscop\u00eda de fluorescencia una morfolog\u00eda nuclear t\u00edpica del mecanismo de muerte celular por apopt\u00f3sis, mientras que con el microscopio electr\u00f3nico de transmisi\u00f3n, aparecen adem\u00e1s, cambios en el citoplasma caracter\u00edsticos de necrosis u oncosis. Mediante la electroforesis d<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Citotoxicidad inducida por cisplatino. estudio experimental in vitro en c\u00e9lulas de feocromocitoma de rata pc-12<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Citotoxicidad inducida por cisplatino. estudio experimental in vitro en c\u00e9lulas de feocromocitoma de rata pc-12 <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Ramos Navas-parejo Juan  M. <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Granada<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 23\/04\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Pascual Vicente Crespo Ferrer<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ignacio P\u00e9rez de vargas ferroni <\/li>\n<li>Jos\u00e9 Manuel Garc\u00eda fern\u00e1ndez (vocal)<\/li>\n<li>Jos\u00e9 Morales l\u00f3pez (vocal)<\/li>\n<li>Antonio Mu\u00f1oz hoyos (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ramos Navas-parejo Juan M. El cisplatino es un agente quimioter\u00e1pico usado ampliamente para el tratamiento de diversos [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[3393,11199,2149,3593,53141,83996],"class_list":["post-29759","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-antonio-munoz-hoyos","tag-ignacio-perez-de-vargas-ferroni","tag-jose-manuel-garcia-fernandez","tag-jose-morales-lopez","tag-pascual-vicente-crespo-ferrer","tag-ramos-navas-parejo-juan-m"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/29759","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=29759"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/29759\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=29759"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=29759"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=29759"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}