{"id":29785,"date":"2018-03-09T09:23:03","date_gmt":"2018-03-09T09:23:03","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/adn-polimerasa-mu-pol-mu-una-enzima-implicada-en-reparacion-y-variabilidad-del-adn\/"},"modified":"2018-03-09T09:23:03","modified_gmt":"2018-03-09T09:23:03","slug":"adn-polimerasa-mu-pol-mu-una-enzima-implicada-en-reparacion-y-variabilidad-del-adn","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/adn-polimerasa-mu-pol-mu-una-enzima-implicada-en-reparacion-y-variabilidad-del-adn\/","title":{"rendered":"Adn polimerasa mu (pol mu), una enzima implicada en reparaci\u00f3n y variabilidad del adn"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jos\u00e9 Francisco Ruiz P\u00e9rez <\/strong><\/h2>\n<p>El objetivo inicial de esta tesis doctoral fue la identificaci\u00f3n de adn polimerasas hom\u00f3logas a la adn polimerasa beta que participaran en procesos de reparaci\u00f3n de adn. As\u00ed, pudimos identificar una nueva adn polimerasa humana perteneciente a la familia x a la que se denomin\u00f3 adn polimerasa mu (pol mu).  como el primer paso para poder demostrar que la pol mu era efectivamente una adn polimerasa, se llev\u00f3 a cabo el clonaje de adnc correspondiente al gen polm humano para introducirlo en vectores de expresi\u00f3n de genes heter\u00f3logos en e.Coli y sobreproducir prote\u00edna recombinante en grandes cantidades.  esta sobre-expresi\u00f3n, junto con un posterior proceso de purificaci\u00f3n selectiva nos permiti\u00f3 obtener elevadas cantidades de pol mu humana recombinante altamente purificada. Con esta prote\u00edna se pudo demostrar in vitro que la pol mu es una adn polimerasa.  tras la demostraci\u00f3n de que la pol mu humana recombinante posee actividad adn polimerasa, se llev\u00f3 a cabo una caracterizaci\u00f3n bioqu\u00edmica detallada de esta enzima, determinando sus par\u00e1metros de eficiencia catal\u00edtica en las reacciones de s\u00edntesis, la afinidad por diferentes tipos de sustratos de adn, la procesividad y la fidelidad de s\u00edntesis. En vista de los an\u00e1lisis preliminares, que indicabn que la pol mu pose\u00eda una baja fidelidad de s\u00edntesis, llevamos a cabo un an\u00e1lisis exhaustivo de la capacidad de incorporaci\u00f3n de errores por pol mu (fenotipo mutador) en diferentes condiciones experimentales. de esta manera pudimos obtener informaci\u00f3n valiosa acerca de c\u00faal es el mecanismo de acci\u00f3n de la pol mu y c\u00f3mo genera los errores de s\u00edntesis.  por otro lado, se llev\u00f3 a cabo un an\u00e1lisis estructura-funci\u00f3n de la pol mu humana, con la finalidad de determinar posibles dominios funcionales y sitio\/s activo\/s de la enzima a lo largo de la estructura primaria de la prote\u00edna. As\u00ed se definieron una serie de res\u00edduos aminoc\u00eddicos supuestamente esenciales en lainterac<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Adn polimerasa mu (pol mu), una enzima implicada en reparaci\u00f3n y variabilidad del adn<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Adn polimerasa mu (pol mu), una enzima implicada en reparaci\u00f3n y variabilidad del adn <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jos\u00e9 Francisco Ruiz P\u00e9rez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 23\/04\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Luis Blanco D\u00e1vila<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: jose Fernandez piqueras <\/li>\n<li>Antonio Bernad miana (vocal)<\/li>\n<li>vicente Larraga rodr\u00edguez de vera (vocal)<\/li>\n<li>Jes\u00fas Bl\u00e1zquez g\u00f3mez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jos\u00e9 Francisco Ruiz P\u00e9rez El objetivo inicial de esta tesis doctoral fue la identificaci\u00f3n de adn polimerasas [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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