{"id":30100,"date":"2018-03-09T09:23:34","date_gmt":"2018-03-09T09:23:34","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aplicacion-de-la-pcr-cuantitativa-en-el-diagnostico-microbiologico-de-patogenos-periodontales\/"},"modified":"2018-03-09T09:23:34","modified_gmt":"2018-03-09T09:23:34","slug":"aplicacion-de-la-pcr-cuantitativa-en-el-diagnostico-microbiologico-de-patogenos-periodontales","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aplicacion-de-la-pcr-cuantitativa-en-el-diagnostico-microbiologico-de-patogenos-periodontales\/","title":{"rendered":"Aplicaci\u00f3n de la pcr cuantitativa en el diagn\u00f3stico microbiol\u00f3gico de pat\u00f3genos periodontales"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Laura Lau Rojo <\/strong><\/h2>\n<p>Los avances en el desarrollo de t\u00e9cnicas de biolog\u00eda molecular, en especial de la pcr (reacci\u00f3n en cadena de la polimerasa), han permitido su aplicaci\u00f3n en el \u00e1mbito diagn\u00f3stico. La reciente aparici\u00f3n de t\u00e9cnicas de cuantificaci\u00f3n en tiempo real mediante pcr ha revolucionado las palicaciones diagn\u00f3sticas de dicha t\u00e9cnica. La cuantificaci\u00f3n de aquellas bacterias involucradas en la aparici\u00f3n de la periodontitis podr\u00eda aporta una informaic\u00f3n diagn\u00f3stica de clara utilidad.  objetivo  optimizar y validar la t\u00e9cnica de pcr cuantitativa en tiempo real mediante sybr green y sondas taqman para la detecci\u00f3n de a.Actinomycetemcomitans (aa), p.Gingivalis (pg) y t.Forythensis (tf) en placa subgingival.  material y m\u00e9todos  partiendo de cepas de tipo, se realiz\u00f3 la optimizaci\u00f3n de sybr green para aa y pg, y de sondas taqman para las tres especies. Se utilizaron primers frente a secuencias de genes de copia \u00fanica y se emple\u00f3 un m\u00e9todo f\u00edsico para la extracci\u00f3n de dna. Se aplic\u00f3 el sistema taqman en 92 muestras procedentes de individuos con salud, gingivitis y periodontitis, las cuales fueron tambi\u00e9n cultivadas para llevar a cabo la comparaci\u00f3n entre ambos m\u00e9todos.  resultados  ambos sistemas de pcr en tiempo real proporcionan una sensibilidad, especificidad y reproducibilidad adecuadas tras la optimizaci\u00f3n. Al comparar pcr y cultivo, se obtuvo una clara divergencia en la preValencia de tf, con un 100% de positivos en periodontitis mediante pcr. Hubo gran concordancia en la cuantificaci\u00f3n de las muestras positivas por ambas t\u00e9cnicas, aunque con valores mayores para cultivo en el caso de pg, y a la inversa en tf.  conclusi\u00f3n  la pcr cuantitativa en tiempo real es un m\u00e9todo sensible, espec\u00edfico y reproducible para la detecci\u00f3n de los tres pat\u00f3genos periodontales en muestras subgingivales.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aplicaci\u00f3n de la pcr cuantitativa en el diagn\u00f3stico microbiol\u00f3gico de pat\u00f3genos periodontales<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aplicaci\u00f3n de la pcr cuantitativa en el diagn\u00f3stico microbiol\u00f3gico de pat\u00f3genos periodontales <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Laura Lau Rojo <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/05\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda no Sanz Alonso<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Antonio Ramos atance <\/li>\n<li>pilar Baca garcia (vocal)<\/li>\n<li>victoriano Serrano cuenca (vocal)<\/li>\n<li> Echevarria Garc\u00eda Jos\u00e9 Javier (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Laura Lau Rojo Los avances en el desarrollo de t\u00e9cnicas de biolog\u00eda molecular, en especial de la [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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