{"id":30263,"date":"2018-03-09T09:23:48","date_gmt":"2018-03-09T09:23:48","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-de-la-expresion-de-un-gen-de-poligalacturonasa-de-alfalfa-en-escherichia-coli-saccharomyces-cerevisiae-schizosaccharomyces-pombe-pichia-pastoris-y-arabidopsis-thaliana\/"},"modified":"2018-03-09T09:23:48","modified_gmt":"2018-03-09T09:23:48","slug":"estudio-de-la-expresion-de-un-gen-de-poligalacturonasa-de-alfalfa-en-escherichia-coli-saccharomyces-cerevisiae-schizosaccharomyces-pombe-pichia-pastoris-y-arabidopsis-thaliana","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/estudio-de-la-expresion-de-un-gen-de-poligalacturonasa-de-alfalfa-en-escherichia-coli-saccharomyces-cerevisiae-schizosaccharomyces-pombe-pichia-pastoris-y-arabidopsis-thaliana\/","title":{"rendered":"Estudio de la expresi\u00f3n de un gen de poligalacturonasa de alfalfa en escherichia coli, saccharomyces cerevisiae, schizosaccharomyces pombe, pichia pastoris y arabidopsis thaliana"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Manuel Prieto Alcedo <\/strong><\/h2>\n<p>La pared celular vegetal es la responsable dela coherencia delos tejidos en plantas. La pared celular est\u00e1 constituida por diversas mol\u00e9culas. Esta formada por una serie de microfibrillas de celulosa embebidas en una matriz formada pro diversos polisac\u00e1ridos (hemicelulosas y pectinas) y glicoprote\u00ednas. las pectinas son un grupo de polisac\u00e1ridos complejos, se localizan en los espacios intercelulares, formando parte de la l\u00e1mina media, y por tanto, son los principales responsables de la coherencia de los tejidos de las plantas. Una de las enzimas de act\u00faan sobre la pectinas son las poligalacturonasas, que hidrolizanla mol\u00e9culas de homogalacturonano, uno de los componentes de las pectinas.  las poligalacturonasas tienen importantes aplicaciones industriales ya que se utilizan para el procesado de frutos para su maceraci\u00f3n y licuefacci\u00f3n previo uso en los procesos industriales. Tambi\u00e9n se emplean para la obtenci\u00f3n de aceites esenciales y carotenoides a partir de las pieles de c\u00edtricos. aunque u su m\u00e1s extendido es la clarificaci\u00f3n, la adici\u00f3n de estas enzimas permite disminuir la viscosidad y obtener mayor cantidad de zumo bebido a una menos colmataci\u00f3n de los filtros. La industria vin\u00edcola tambi\u00e9n las emplean, a\u00f1adi\u00e9ndolas al comienzo de la fermentaci\u00f3n.  en esta tesis se ha estudiado una poligalacturonasa de alfalfa implicada en el proceso de simbiosis entre la planta y rhizobium, el gen mspg3. Se clon\u00f3 este gen para su expresi\u00f3n en bacterias y levaduras saccharomyces cerevisiae, schizosaccharomyces pombe y pichia pastoris. En todos los organismos empleados se detect\u00f3 la producci\u00f3n de cierta cantidad de enzima, pero la cantidad es tan peque\u00f1a que el empleo de estas estirpes transformadas para obtener la enzima en grandes cantidades no es viable.  otro de los objetivos marcados en la tesis ha sido el efecto del estudio dela sobreexpresi\u00f3n del gen en plantas. Se emple\u00f3 arabidopsis thaliana debido a que es la pla<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio de la expresi\u00f3n de un gen de poligalacturonasa de alfalfa en escherichia coli, saccharomyces cerevisiae, schizosaccharomyces pombe, pichia pastoris y arabidopsis thaliana<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio de la expresi\u00f3n de un gen de poligalacturonasa de alfalfa en escherichia coli, saccharomyces cerevisiae, schizosaccharomyces pombe, pichia pastoris y arabidopsis thaliana <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Manuel Prieto Alcedo <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Santiago de compostela<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 21\/05\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Tom\u00e1s Gonz\u00e1lez Villa<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Miguel Vi\u00f1as ciordia <\/li>\n<li>Jos\u00e9 Cansado vizoso (vocal)<\/li>\n<li>pedro Mart\u00ednez jose (vocal)<\/li>\n<li>julio ernesto Abalde alonso (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Manuel Prieto Alcedo La pared celular vegetal es la responsable dela coherencia delos tejidos en plantas. La [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[494,35,977],"tags":[62193,21217,85144,1241,85145,2553],"class_list":["post-30263","post","type-post","status-publish","format-standard","hentry","category-ciencias-agrarias","category-ciencias-de-la-vida","category-santiago-de-compostela","tag-jose-cansado-vizoso","tag-julio-ernesto-abalde-alonso","tag-manuel-prieto-alcedo","tag-miguel-vinas-ciordia","tag-pedro-Martinez-jose","tag-tomas-gonzalez-villa"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/30263","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=30263"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/30263\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=30263"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=30263"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=30263"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}