{"id":30527,"date":"2004-04-06T00:00:00","date_gmt":"2004-04-06T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/maximal-transduction-capability-of-akv-mlv-based-vectors-lengthened-genomes-and-their-replication-does-size-matter\/"},"modified":"2004-04-06T00:00:00","modified_gmt":"2004-04-06T00:00:00","slug":"maximal-transduction-capability-of-akv-mlv-based-vectors-lengthened-genomes-and-their-replication-does-size-matter","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/maximal-transduction-capability-of-akv-mlv-based-vectors-lengthened-genomes-and-their-replication-does-size-matter\/","title":{"rendered":"Maximal transduction capability of akv-mlv-based vectors  lengthened genomes and their replication, does size matter?"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Carrasco Cornejo M. Luisa <\/strong><\/h2>\n<p>Durante las \u00faltimas d\u00e9cadas, los vectores de transferencia g\u00e9nica m\u00e1s usandos en terapia g\u00e9nica humana han sido basados en diferentes tipos de retrovirus. estos vectores presentan, sin embargo, una capaciedad limitada en cuanto al tama\u00f1o del transg\u00e9n que puede alojar. La presente tesis doctoral se centra en el estudio de la capacidad maxima de transducci\u00f3n de vectores basados en el virus de la leucemia murina akv para su posible utilizaci\u00f3n en terapia g\u00e9nica.  se han construido varios vectores basados en el virus akv conteniendo genomas de tama\u00f1os crecientes, y se ha analizado su capacidad de replicaci\u00f3n en function del tama\u00f1o gen\u00f3mico. Se ha utilizado para ello una t\u00e9cnica (sistema de maxi-virus) desarrollada y reportada en nuestro laboratorio en 1999, que se basa en la inserci\u00f3n, en la regi\u00f3n u3 del ltr, de un ires que dirige la expressi\u00f3n del gen testigo neo insertado a continuaci\u00f3n (virus akvbi-neo). a partir de este virus, y mediante la inserci\u00f3n en su parte previa de fragmentos de tama\u00f1o creciente de dna del fago lambda se generaron mega-vectores cuya capacidad de transducci\u00f3n y estabilidad fueron estudiados. Cuando el tama\u00f1o del genoma viral sobrepas\u00f3 las 10 kb, la eficiencia de transducci\u00f3n decreci\u00f3 de 10 5 to 10 1 dfu\/ml, dependiendo del tama\u00f1o del inserto.  en este trabajo se ha analizado los distintos pasos en el ciclo de replicaci\u00f3n para determinar en que estado\/s son deficientes estos vectores y as\u00ed explicar la reducci\u00f3n obtenida en la capacidad de transducci\u00f3n.  la segunda parte de la tesis se centra en el estudio un maxi-virus quim\u00e9rico, en el cual el promotor propio del virus akv se ha sustituido por el correspondiente al virus sl3-3-mlv. Este maxi-virus contiene un \u00abcassette\u00bb (formado por el ires seguido del gen egfp), insertado en la regi\u00f3n u3 del ltr. Se han analizado los cambios tanto en la replicaci\u00f3n del vector como en la expresi\u00f3n del gen testigo durante seis pases del virus. En l<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Maximal transduction capability of akv-mlv-based vectors  lengthened genomes and their replication, does size matter?<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Maximal transduction capability of akv-mlv-based vectors  lengthened genomes and their replication, does size matter? <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Carrasco Cornejo M. Luisa <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 04\/06\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Finn Skou Pedersen<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan Ortin monton <\/li>\n<li>marc Piechaczyk (vocal)<\/li>\n<li>dieter Klein (vocal)<\/li>\n<li>Jos\u00e9 Alcami pertejo (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carrasco Cornejo M. Luisa Durante las \u00faltimas d\u00e9cadas, los vectores de transferencia g\u00e9nica m\u00e1s usandos en terapia [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[85726,85729,85727,22623,14725,85728],"class_list":["post-30527","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-carrasco-cornejo-m-luisa","tag-dieter-klein","tag-finn-skou-pedersen","tag-jose-alcami-pertejo","tag-juan-ortin-monton","tag-marc-piechaczyk"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/30527","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=30527"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/30527\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=30527"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=30527"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=30527"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}