{"id":30841,"date":"2018-03-09T09:24:38","date_gmt":"2018-03-09T09:24:38","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-termodinamico-y-cinetico-de-la-interacion-entre-intercalantes-y-acidos-nucleicos\/"},"modified":"2018-03-09T09:24:38","modified_gmt":"2018-03-09T09:24:38","slug":"estudio-termodinamico-y-cinetico-de-la-interacion-entre-intercalantes-y-acidos-nucleicos","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/burgos\/estudio-termodinamico-y-cinetico-de-la-interacion-entre-intercalantes-y-acidos-nucleicos\/","title":{"rendered":"Estudio termodinamico y cinetico de la interacion entre intercalantes y acidos nucleicos"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Vittorio Paiotta <\/strong><\/h2>\n<p>Este proyecto de investigaci\u00f3n aborda el estudio de la intercalaci\u00f3n de unos ligandos con una mol\u00e9cula de arn sint\u00e9tico en forma de triple h\u00e9lice. el arn utilizado es el poly(a)opoly(2u), en el cual est\u00e1n presentes dos cadenas de \u00e1cido poliurid\u00edlico [poly(u)], y una de \u00e1cido poliaden\u00ednico [poly(a)].  las reacciones objeto de estudio fueron investigadas por v\u00eda termodin\u00e1mica y cin\u00e9tica. Se pueden aprovechar las caracter\u00edsticas de los reactivos descritas anteriormente para realizar valoraciones espectrofotom\u00e9tricas y fluorim\u00e9tricas, a\u00f1adiendo un ligando a una disoluci\u00f3n de \u00e1cido nucl\u00e9ico y observando la variaci\u00f3n del espectro; esa variaci\u00f3n es caracter\u00edstica de los reactivos en estudio y permite determinar la constante de equilibrio de la reacci\u00f3n. analizando los datos con los m\u00e9todos matem\u00e1ticos de scatchard y mcghee-von hippel pudo medirse el n\u00famero de cavidades del pol\u00edmero inactivadas por la intercalaci\u00f3n de una mol\u00e9cula de bromuro de etidio en condiciones de saturaci\u00f3n. Adem\u00e1s, se pudieron encontrar datos cuantitativos sobre el tipo de cooperatividad  de este proceso de reacci\u00f3n. La presencia del ligando, en efecto, altera la estructura de la triple h\u00e9lice del arn, y modifica las caracter\u00edsticas qu\u00edmicas. Como cabe esperar, la cooperatividad result\u00f3 ser  negativa, es decir, una mol\u00e9cula de bromuro de etidio que se intercala en el \u00e1cido nucl\u00e9ico, desfavorece la intercalaci\u00f3n de otras mol\u00e9culas del mismo ligando.  informaciones de tipo qu\u00edmico f\u00edsico (temperatura de paso desde triple a doble, y desde doble a h\u00e9lice simple) fueron tambi\u00e9n detectadas a partir de medidas de desnaturalizaci\u00f3n t\u00e9rmica del pol\u00edmero y del complejo pol\u00edmero- ligando.  las t\u00e9cnicas utilizadas para la investigaci\u00f3n de la cin\u00e9tica del proceso de reacci\u00f3n fueron t-jump, y stopped-flow. En esta \u00faltima t\u00e9cnica se mezclan los dos reactivos en una c\u00e1mara de mezcla por la que pasa una radiaci\u00f3n monocrom\u00e1tica,<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio termodinamico y cinetico de la interacion entre intercalantes y acidos nucleicos<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio termodinamico y cinetico de la interacion entre intercalantes y acidos nucleicos <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Vittorio Paiotta <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Burgos<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/06\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Fernando Secco<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: julio Casado linarejos <\/li>\n<li>Francisco Sanchez burgos (vocal)<\/li>\n<li>Manuel Cortijo merida (vocal)<\/li>\n<li>marcella Venturini (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Vittorio Paiotta Este proyecto de investigaci\u00f3n aborda el estudio de la intercalaci\u00f3n de unos ligandos con una [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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