{"id":30856,"date":"2018-03-09T09:24:39","date_gmt":"2018-03-09T09:24:39","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/interaccion-de-la-protea%c2%adna-repa-con-el-dna-del-plasmido-pps10-claves-sobre-la-activacion-conformacional-de-un-iniciador-de-la-replicacion\/"},"modified":"2018-03-09T09:24:39","modified_gmt":"2018-03-09T09:24:39","slug":"interaccion-de-la-protea%c2%adna-repa-con-el-dna-del-plasmido-pps10-claves-sobre-la-activacion-conformacional-de-un-iniciador-de-la-replicacion","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/interaccion-de-la-protea%c2%adna-repa-con-el-dna-del-plasmido-pps10-claves-sobre-la-activacion-conformacional-de-un-iniciador-de-la-replicacion\/","title":{"rendered":"Interacci\u00f3n de la prote\u00edna repa con el dna del pl\u00e1smido pps10: claves sobre la activaci\u00f3n conformacional de un iniciador de la replicaci\u00f3n"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Teresa D\u00edaz L\u00f3pez <\/strong><\/h2>\n<p>Repa es la prote\u00edna iniciadora de la replicaci\u00f3n del pl\u00e1smido de pseudomonas pps10. En un rango de concentraci\u00f3n del orden de micromolar, repa existe como d\u00edmero en disoluci\u00f3n. Los d\u00edmeros se unen a dos repeticiones invertidas localizadas en el promotor del gen repa, reprimiendo su propia s\u00edntesis. los mon\u00f3meros, sin embargo, se unen a cuatro secuencias directamente repetidas del origen (denominadas iterones) iniciando replicaci\u00f3n. Repa est\u00e1 compuesta de dos dominios \u00ab\u00bbwinged-helix (wh).  para unirse a la secuencia del iter\u00f3n a trav\u00e9s de los dos dominios. Repa debe acoplar la monomerizaci\u00f3n con un cambio conformacional en el dominio n-terminal (wh1), que incluye un motivo cremallera de leucinas. En esta tesis se muestra por primera vez que la secuencia del iter\u00f3n, in vitro, disocia los d\u00edmeros en mon\u00f3meros y altera la conformaci\u00f3n de repa, sugiriendo un efecto alost\u00e9rico. La informaci\u00f3n estructural reciente sobre el d\u00edmero de repa, junto con la estructura de la forma monom\u00e9rica de la prote\u00edna hom\u00f3loga repe del pl\u00e1smido f, muestran las bases moleculares de la activaci\u00f3n de prote\u00ednas rep. En este trabajo se caracterizan mutaciones que sustituyen dos residuos de leu por ala en el motivo cremallera de leucinas de repa. este mutante, denominado repa-2l2a, que desetabiliza el n\u00facleo hidrof\u00f3bico del wh1, resulta en cambios estructurales que se asemejan a los que ocurren en la activaci\u00f3n de la prote\u00edna. Proponemos en esta tesis que repa-2l2a se asemeja a un intermediario de plegamiento en la ruta de obtenci\u00f3n de mon\u00f3meros activos.  en este trabajo, se ha observado que interacciones de baja afinidad de la prote\u00edna repa con las secuencias del operador y del iter\u00f3n, se favorecen utilizando altas concentraciones (hasta 50 mg\/ml) de bsa, ovoalb\u00famina y mioglobina. Estos aditivos proteicos, que ocupan una fracci\u00f3n significativa del medio y no participan directamente en la interacci\u00f3n, mejoran la uni\u00f3n inicial de la<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Interacci\u00f3n de la prote\u00edna repa con el dna del pl\u00e1smido pps10: claves sobre la activaci\u00f3n conformacional de un iniciador de la replicaci\u00f3n<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Interacci\u00f3n de la prote\u00edna repa con el dna del pl\u00e1smido pps10: claves sobre la activaci\u00f3n conformacional de un iniciador de la replicaci\u00f3n <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Teresa D\u00edaz L\u00f3pez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 18\/06\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Rafael Giraldo Su\u00e1rez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: armando Garrido pertierra <\/li>\n<li>gloria Del solar  dongil (vocal)<\/li>\n<li>Jos\u00e9 Miguel Hermoso  nu\u00f1ez (vocal)<\/li>\n<li>enrique Viguera m\u00ednguez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Teresa D\u00edaz L\u00f3pez Repa es la prote\u00edna iniciadora de la replicaci\u00f3n del pl\u00e1smido de pseudomonas pps10. En [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[86450,64806,58467,14480,51887,86449],"class_list":["post-30856","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-armando-garrido-pertierra","tag-enrique-viguera-minguez","tag-gloria-del-solar-dongil","tag-jose-miguel-hermoso-nunez","tag-rafael-giraldo-suarez","tag-teresa-diaz-lopez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/30856","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=30856"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/30856\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=30856"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=30856"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=30856"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}