{"id":31848,"date":"1997-01-01T00:00:00","date_gmt":"1997-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-cristalografico-del-complejo-dcgcaaatttgcg2-hoechst-33258-y-de-tres-proteinas-cm-gst-p-tcmip-y-xylanasa-a\/"},"modified":"1997-01-01T00:00:00","modified_gmt":"1997-01-01T00:00:00","slug":"estudio-cristalografico-del-complejo-dcgcaaatttgcg2-hoechst-33258-y-de-tres-proteinas-cm-gst-p-tcmip-y-xylanasa-a","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/estudio-cristalografico-del-complejo-dcgcaaatttgcg2-hoechst-33258-y-de-tres-proteinas-cm-gst-p-tcmip-y-xylanasa-a\/","title":{"rendered":"Estudio cristalografico del complejo d(cgcaaatttgcg)2-hoechst 33258 y de tres proteinas: cm-gst-p, tcmip y xylanasa a."},"content":{"rendered":"<h2>Tesis doctoral de <strong> M. Cristina Vega Fernandez <\/strong><\/h2>\n<p>El objetivo de esta tesis doctoral es la determinacion estructural por difraccion de rayos x de cuatro compuestos: un complejo dna-droga y tres proteinas. La metodolog\u00eda usada (la cristalografia de monocristal de biomoleculas) consta principalmente de las siguientes etapas:  1- la obtencion de monocristales mediante tecnicas relacionadas con la bioquimica y la ingenieria genetica (clonaje, expresion y purificacion de proteinas).  2.- La recogida de los datos de difraccion que en muchos casos necesita llevarse a cabo con radiacion de sincrotron.  3.- El tratamiento de los datos de difraccion que requiere el uso de grandes computadores y de paquetes informaticos especialmente dise\u00f1ados para ello.  los resultados obtenidos para cada molecula estudiada son:  1) complejo d(cgcaaatttgcg)2- hoechst 33258:  determinacion de la estructura tridimensional del complejo adn &#8211; droga y comparacion con otros complejos relacionados, con la estructura del adn solo y de la droga sola.  2) gst-pi modificada en la cys47: determinacion de la estructura de la primera forma apo de esta enzima y del complejo formado con el inhibidor s-(p-nitrobencil)glutation, lo que permite establecer un mecanismo enzimatico.  3) tcmip: cristalizacion de esta proteina, obtencion de derivados pesados, recoleccion de datos de difraccion de ambos y obtencion de las fases iniciales.  4) xylanasa a: cristalizacion de esta proteina y recoleccion de los datos de difraccion.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio cristalografico del complejo d(cgcaaatttgcg)2-hoechst 33258 y de tres proteinas: cm-gst-p, tcmip y xylanasa a.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio cristalografico del complejo d(cgcaaatttgcg)2-hoechst 33258 y de tres proteinas: cm-gst-p, tcmip y xylanasa a. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 M. Cristina Vega Fernandez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de catalunya<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1997<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Miquel Coll Capella<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  Antonio Subirana Torrent <\/li>\n<li>Francisco Javier Luque Garriga (vocal)<\/li>\n<li>Ignacio Fita Rodriguez (vocal)<\/li>\n<li>Carlos Miravitlles Torras (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de M. Cristina Vega Fernandez El objetivo de esta tesis doctoral es la determinacion estructural por difraccion de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[160,115,396,15596,17,966],"tags":[12155,39694,15733,117,88439,26322],"class_list":["post-31848","post","type-post","status-publish","format-standard","hentry","category-bioquimica","category-bioquimica-molecular","category-polipeptidos-y-proteinas","category-politecnica-de-catalunya","category-quimica","category-quimica-macromolecular","tag-carlos-miravitlles-torras","tag-francisco-javier-luque-garriga","tag-ignacio-fita-rodriguez","tag-juan-antonio-subirana-torrent","tag-m-cristina-vega-fernandez","tag-miquel-coll-capella"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/31848","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=31848"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/31848\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=31848"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=31848"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=31848"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}