{"id":33310,"date":"2018-03-09T09:30:22","date_gmt":"2018-03-09T09:30:22","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/mecanismo-de-represion-del-promotor-temprano-a2c-por-la-proteina-p4-del-bacteriofago-029-de-b-subtilis\/"},"modified":"2018-03-09T09:30:22","modified_gmt":"2018-03-09T09:30:22","slug":"mecanismo-de-represion-del-promotor-temprano-a2c-por-la-proteina-p4-del-bacteriofago-029-de-b-subtilis","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/mecanismo-de-represion-del-promotor-temprano-a2c-por-la-proteina-p4-del-bacteriofago-029-de-b-subtilis\/","title":{"rendered":"Mecanismo de represion del promotor temprano a2c por la proteina p4 del bacteriofago 029 de b. subtilis."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Mar\u00eda Monsalve P\u00e9rez <\/strong><\/h2>\n<p>Partiendo de la observaci\u00f3n de que la prote\u00edna p4 pod\u00eda regular actividad del promotor a2c tanto in vivo como in vitro, se inici\u00f3 caracterizaci\u00f3n del mecanismo de represi\u00f3n empezando por la determinaci\u00f3n del punto del proceso de inicio de la transcripci\u00f3n sobre el que actuaba la prote\u00edna p4 en el promotor a2c, que result\u00f3 ser el proceso de salida o escape de la rnap del promotor. Se observ\u00f3 seguidamente que el mecanismo de represi\u00f3n operaba a trav\u00e9s de la interacci\u00f3n directa entre el extremo c-terminal de la prote\u00edna p4. Y el dominio ctd de la subunidad alfa de la rnap. La construcci\u00f3n de variantes en el promotor a2c y en el promotor a3, este \u00faltimo activable por p4, llev\u00f3 a la conclusi\u00f3n de que el efecto regulador de p4 sobre un promotor puede ser tanto activador o represor, dependiendo \u00fanicamente de la fuerza de las interacciones de la rnap con las secuencias promotoras. Finalmente, se caracteriz\u00f3 el sitio de uni\u00f3n de p4 en el promotor a2c responsable de su capacidad reguladora.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Mecanismo de represion del promotor temprano a2c por la proteina p4 del bacteriofago 029 de b. subtilis.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Mecanismo de represion del promotor temprano a2c por la proteina p4 del bacteriofago 029 de b. subtilis. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Mar\u00eda Monsalve P\u00e9rez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 20\/11\/1997<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Margarita Salas Falgueras<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: tom\u00e1s Ruiz de argueso <\/li>\n<li>Antonio Tormo garrido (vocal)<\/li>\n<li>Manuel Espinosa padr\u00f3n (vocal)<\/li>\n<li>Juan  Luis Ramos martin (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Mar\u00eda Monsalve P\u00e9rez Partiendo de la observaci\u00f3n de que la prote\u00edna p4 pod\u00eda regular actividad del promotor [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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