{"id":33782,"date":"1998-01-01T00:00:00","date_gmt":"1998-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/protein-quinasa-c-en-rodaballo-scophthalmus-maximus-caracterizacion-y-expresion-en-tejidos\/"},"modified":"1998-01-01T00:00:00","modified_gmt":"1998-01-01T00:00:00","slug":"protein-quinasa-c-en-rodaballo-scophthalmus-maximus-caracterizacion-y-expresion-en-tejidos","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/protein-quinasa-c-en-rodaballo-scophthalmus-maximus-caracterizacion-y-expresion-en-tejidos\/","title":{"rendered":"Protein quinasa c en rodaballo (scophthalmus maximus). caracterizacion y expresion en tejidos."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Puente Novoa Jos\u00e9 Manuel <\/strong><\/h2>\n<p>En este trabajo, se ha aislado una actividad prote\u00edn quinasa c a partir de bazo de rodaballo mediante tres pasos cromatogr\u00e1ficos: deae, fenil-sefarosa y treon\u00edn-sefarosa. La actividad era calcio-fosfol\u00edpido dependiente, se inhib\u00eda por h7 y en western blot se obten\u00edan dos bandas, una de 80 y otra de 100 kda. Con el monoclonal mc5. Las concentraciones \u00f3ptimas de activadores para su plena actividad eran 0.1 mm calcio, 20 microg\/ml fosfatidilserina y 2 microg\/ml diacilglicerol. La pkc purificada fosforil\u00f3 con alta eficiencia dos sustratos espec\u00edficos: marcks151-175 y egfr651-658, y fue inhibida espec\u00edficamente por dos p\u00e9ptidos pseudosustrato: pkc19-36 y pkc19-31. Por western blot, se demostr\u00f3 que la actividad purificada conten\u00eda seis isoformas: alfa, beta, delta, epsilon, zeta y lambda, mientras que en extracto crudo de bazo se detectaron las seis anteriores, mu y theta. Alfa presentaba dos formas de distinto peso molecular, una de 80 y otra de 100 kda. Cargando la actividad purificada en una mono q, se obtuvieron dos picos de actividad, en uno de los cuales est\u00e1n presentes delta y lambda. El an\u00e1lisis de la expresi\u00f3n de isoformas de pkc en diferentes tejidos de rodaballo: h\u00edgado, g\u00f3nada, m\u00fasculo, coraz\u00f3n, cerebro, intestino, aleta caudal y ri\u00f1\u00f3n; demostr\u00f3 que las isoformas alfa (con una masa de 100 kda.), Beta, delta, epsilon, lambda y mu, est\u00e1n presentes en todos los tejidos. Zeta se detect\u00f3 en todos salvo en m\u00fasculo, mientras que gamma y theta est\u00e1n presentes en cerebro y en m\u00fasculo y cerebro, respectivamente.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Protein quinasa c en rodaballo (scophthalmus maximus). caracterizacion y expresion en tejidos.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Protein quinasa c en rodaballo (scophthalmus maximus). caracterizacion y expresion en tejidos. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Puente Novoa Jos\u00e9 Manuel <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Santiago de compostela<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1998<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Manuel Rey M\u00e9ndez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Germ\u00e1n Sierra Marcu\u00f1o <\/li>\n<li>Ignacio Ramos Martinez (vocal)<\/li>\n<li> Barja Perez Juan  Luis (vocal)<\/li>\n<li>Amando Garrido Pertierra (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Puente Novoa Jos\u00e9 Manuel En este trabajo, se ha aislado una actividad prote\u00edn quinasa c a partir [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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