{"id":34051,"date":"1998-01-01T00:00:00","date_gmt":"1998-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/el-complejo-de-traslocacion-de-preproteina-de-streptomyces-lividans-1326-analisis-de-los-genes-seca-sece-y-secy\/"},"modified":"1998-01-01T00:00:00","modified_gmt":"1998-01-01T00:00:00","slug":"el-complejo-de-traslocacion-de-preproteina-de-streptomyces-lividans-1326-analisis-de-los-genes-seca-sece-y-secy","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/el-complejo-de-traslocacion-de-preproteina-de-streptomyces-lividans-1326-analisis-de-los-genes-seca-sece-y-secy\/","title":{"rendered":"El complejo de traslocacion de preproteina de streptomyces lividans 1326. analisis de los genes seca, sece y secy."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jorge Blanco Menda\u00f1a <\/strong><\/h2>\n<p>Las prote\u00ednas seca, sece y secy son componentes altamente conservados de la maquinaria de exportaci\u00f3n de prote\u00ednas bacteriana. Sus genes codificantes (seca, sece y secy, respectivamente), identificados inicialmente en escherichia coli, han sido localizados en una amplia variedad de microorganismos, entre ellos la bacteria streptomyces lividans (este trabajo).  se ha llevado a cabo la sobreexpresi\u00f3n hom\u00f3loga del gen seca por aumento de la dosis g\u00e9nica y por sustituci\u00f3n de su promotor (pseca) por promotor psaf (uno de los promotores m\u00e1s fuertes conocidos en streptomyces). A partir de la cepa sobreproductora, se ha realizado la purificaci\u00f3n de la prote\u00edna seca y su caracterizaci\u00f3n bioqu\u00edmica: determinaci\u00f3n de sus actividades atpasas, estudio de su capacidad de unir nucle\u00f3tidos, an\u00e1lisis de su cin\u00e9tica de uni\u00f3n a membranas de streptomyces y determinaci\u00f3n de su incapacidad para interaccionar con el chaper\u00f3n molecular de e. Coli secb.  se ha llevado a cabo tambi\u00e9n la sobreexpresi\u00f3n hom\u00f3loga de los genes sece y secy (cada uno con su propio promotor) por aumento de la dosis g\u00e9nica. La prote\u00edna secy de s. Lividans fue reconocida por anticuerpos anti-secy de bacillus subtilis. Sin embargo, sece no reaccion\u00f3 frente a anticuerpos anti-sece de b. Subtilis ni anti-sece de e. Coli.  la expresi\u00f3n de los genes seca, sece y secy de s.  lividans en mutantes de cepas de e. Coli adecuadas, permiti\u00f3 determinar que las prote\u00ednas codificadas por ellos carec\u00edan de funcionalidad en e. Coli y, adem\u00e1s, sece y secy resultaban t\u00f3xicas para la viabilidad de las c\u00e9lulas de e. Coli.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>El complejo de traslocacion de preproteina de streptomyces lividans 1326. analisis de los genes seca, sece y secy.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 El complejo de traslocacion de preproteina de streptomyces lividans 1326. analisis de los genes seca, sece y secy. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jorge Blanco Menda\u00f1a <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1998<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Francisco Mart\u00edn Mart\u00edn<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Carlos Hardisson Rumeu <\/li>\n<li>Ram\u00f3n Santamar\u00eda S\u00e1nchez (vocal)<\/li>\n<li>Mar\u00eda Enriqueta Arias Fern\u00e1ndez (vocal)<\/li>\n<li>Jes\u00fas Fernandez Aparicio (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jorge Blanco Menda\u00f1a Las prote\u00ednas seca, sece y secy son componentes altamente conservados de la maquinaria de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[35,249,4430,278],"tags":[9056,36063,92276,4494,9364,9093],"class_list":["post-34051","post","type-post","status-publish","format-standard","hentry","category-ciencias-de-la-vida","category-fisiologia-bacteriana","category-leon","category-microbiologia","tag-carlos-hardisson-rumeu","tag-jesus-fernandez-aparicio","tag-jorge-blanco-mendana","tag-juan-francisco-martin-martin","tag-maria-enriqueta-arias-fernandez","tag-ramon-santamaria-sanchez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/34051","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=34051"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/34051\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=34051"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=34051"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=34051"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}