{"id":35678,"date":"1998-01-01T00:00:00","date_gmt":"1998-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/clonacion-y-estructura-tridimensional-de-la-carbamato-quinasa-cristalizacion-d-e-la-carbamilfosfato-sintetasa-i\/"},"modified":"1998-01-01T00:00:00","modified_gmt":"1998-01-01T00:00:00","slug":"clonacion-y-estructura-tridimensional-de-la-carbamato-quinasa-cristalizacion-d-e-la-carbamilfosfato-sintetasa-i","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/clonacion-y-estructura-tridimensional-de-la-carbamato-quinasa-cristalizacion-d-e-la-carbamilfosfato-sintetasa-i\/","title":{"rendered":"Clonacion y estructura tridimensional de la carbamato quinasa. cristalizacion d e la carbamilfosfato sintetasa i."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Alberto Marina Moreno <\/strong><\/h2>\n<p>El trabajo de tesis describe la purificaci\u00f3n de la enzima carmato quinasa producida por enterococcus faecium, el clonado y secuenciaci\u00f3n del gen que codifica para esta prote\u00edna en enterococcus faecium y enterococcus faecalis, su subclonado en escherichia coli, la expresi\u00f3n y purificaci\u00f3n de dicha enzima producida a partir de estos clones de e. Coli, la cristalizaci\u00f3n de la carbamato quinasa natural o, recombinante, y la determinaci\u00f3n de la estructura tridimensional de esta enzima a 2.8 a de resoluci\u00f3n utilizando t\u00e9cnicas de difracci\u00f3n de rayos x.  la carbamato quinasa es un d\u00edmero, constituido por mon\u00f3meros de 310 amino\u00e1cidos, cuyo elemento estructural principal es una hoja beta abierta de 8 elementos en su mayor\u00eda paralelos, rodeada por ambas caras de alfa h\u00e9lices. En esta hoja beta se observa una gran oquedad, donde se ha localizado un i\u00f3n sulfato, y que se propone como centro activo de la enzima.  tambi\u00e9n se describe la purificaci\u00f3n y cristalizaci\u00f3n de la carbamilfosfato sintetasa i de rana catesbeiana y la caracterizaci\u00f3n de estos cristales usando t\u00e9cnicas de rayos x.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Clonacion y estructura tridimensional de la carbamato quinasa. cristalizacion d e la carbamilfosfato sintetasa i.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Clonacion y estructura tridimensional de la carbamato quinasa. cristalizacion d e la carbamilfosfato sintetasa i. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Alberto Marina Moreno <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Universitat de val\u00e9ncia (estudi general)<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1998<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Vicente Rubio Zamora<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Santiago Grisolia Garcia <\/li>\n<li>Francesc Xavier Avil\u00e9s Puigvert (vocal)<\/li>\n<li>Pedro Alzari (vocal)<\/li>\n<li>Carlos Gomez-moreno Calera (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Alberto Marina Moreno El trabajo de tesis describe la purificaci\u00f3n de la enzima carmato quinasa producida por [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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