{"id":36944,"date":"1998-01-01T00:00:00","date_gmt":"1998-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/purificacion-clonacion-expresion-y-propiedades-inhibitorias-de-una-cistatina-de-castanea-sativa\/"},"modified":"1998-01-01T00:00:00","modified_gmt":"1998-01-01T00:00:00","slug":"purificacion-clonacion-expresion-y-propiedades-inhibitorias-de-una-cistatina-de-castanea-sativa","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/purificacion-clonacion-expresion-y-propiedades-inhibitorias-de-una-cistatina-de-castanea-sativa\/","title":{"rendered":"Purificacion, clonacion, expresion y propiedades inhibitorias de una cistatina de castanea sativa."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Monica Pernas Ochoa <\/strong><\/h2>\n<p>Se ha llevado a cabo la purificaci\u00f3n y caracterizaci\u00f3n de un inhibidor de ciste\u00edn proteasas, csc, de semilla de c.  sativa. Se ha aislado su cl\u00f3n de cdna a partir de una librer\u00eda de semillas inmaduras de casta\u00f1a. El inhibidor fu\u00e9 expresado en e. Coli y purificado del extracto bacteriano. La identidad de la cistatina recombinante y la de semilla fue confirmada por analisis maldi, electroforesis bidimensional y las secuencias amino terminales. La csc tiene una masa molecular de 11275 da y un pi de 6.9. Su consecuencia de amino\u00e1cidos incluye tres motivos conservados esenciales para la actividad inhibitoria y muestra una identidad de secuencia significativa con otras fitocistatinas. La csc recombinante inhibe papa\u00edna (ki:29nm), ficina (ki: 65 nm), quimopapa\u00edna (ki: 366nm) y catepsina b (ki: 473nm).  al contrario de la mayor\u00eda de las cistatinas tambi\u00e9n se detect\u00f3 actividad inhibitoria frente a tripsina (ki:3489 nm). Los patrones de expresi\u00f3n de csc durante el desarrollo de la semilla fueron diferentes a los de otras cistatinas. La csc es efectiva frente a extractos de distintos \u00e1caros e insectos.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Purificacion, clonacion, expresion y propiedades inhibitorias de una cistatina de castanea sativa.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Purificacion, clonacion, expresion y propiedades inhibitorias de una cistatina de castanea sativa. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Monica Pernas Ochoa <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1998<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Gabriel Salcedo Dur\u00e1n<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Cipriano Aragoncillo Ballesteros <\/li>\n<li>Julio Salinas Mu\u00f1oz (vocal)<\/li>\n<li>Pedro Casta\u00f1era Dominguez (vocal)<\/li>\n<li>Jos\u00e9 Juan Sanchez Serrano (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Monica Pernas Ochoa Se ha llevado a cabo la purificaci\u00f3n y caracterizaci\u00f3n de un inhibidor de ciste\u00edn [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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