{"id":38090,"date":"2018-03-09T09:37:30","date_gmt":"2018-03-09T09:37:30","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/purificacion-y-caracterizacion-del-receptor-nuclear-de-la-melatonina-en-hepatocitos-de-rata\/"},"modified":"2018-03-09T09:37:30","modified_gmt":"2018-03-09T09:37:30","slug":"purificacion-y-caracterizacion-del-receptor-nuclear-de-la-melatonina-en-hepatocitos-de-rata","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/purificacion-y-caracterizacion-del-receptor-nuclear-de-la-melatonina-en-hepatocitos-de-rata\/","title":{"rendered":"Purificacion y caracterizacion del receptor nuclear de la melatonina en hepatocitos de rata."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Manuel Macias Gonzalez <\/strong><\/h2>\n<p>El objetivo principal de esta tesis ha sido la purificaci\u00f3n y caracterizaci\u00f3n del receptor nuclear de la melatonina en hepatocitos de rata.  para ello se aislaron los n\u00facleos y se investig\u00f3 la localizaci\u00f3n nuclear de la melatonina en hepatocitos de rata, mediante t\u00e9cnicas de microscop\u00eda electr\u00f3nica y citometr\u00eda de flujo. En segundo lugar, se purific\u00f3 el receptor de la melatonina principalmente mediante cromatograf\u00eda de afinidad. Finalmente, se caracteriz\u00f3 el receptor nuclear de la melatonina mediante t\u00e9cnicas de autorradiograf\u00eda, electroforesis e inmunotransferencia.  la identificaci\u00f3n del receptor nuclear de la melatonina se consigui\u00f3 mediante el uso de ligandos espec\u00edficos para \u00e9ste.  por t\u00e9cnicas de inmunocitoqu\u00edmica y citometr\u00eda de flujo se demostr\u00f3 la presencia de melatonina en dichos n\u00facleos.  durante el proceso de purificaci\u00f3n del receptor se obtuvo el m\u00e1ximo rendimiento tratando el homogeneizado de n\u00facleos con 25-65% de sulfato am\u00f3nico, siendo esta fracci\u00f3n la utilizada en cromatograf\u00eda afinidad. La caracterizaci\u00f3n de las prote\u00ednas purificadas determin\u00f3 la presencia de dos prote\u00ednas que se un\u00edan especificamente a la melatonina de 64 y 74 kda respectivamente.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Purificacion y caracterizacion del receptor nuclear de la melatonina en hepatocitos de rata.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Purificacion y caracterizacion del receptor nuclear de la melatonina en hepatocitos de rata. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Manuel Macias Gonzalez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Granada<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 24\/04\/1998<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Dario Acu\u00f1a Castroviejo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal:  Guerrero mont\u00e1lvez Juan  Miguel <\/li>\n<li>carmen Rodriguez sanchez (vocal)<\/li>\n<li>Jos\u00e9 Manuel Baeyens cabrera (vocal)<\/li>\n<li>carstern Carlberg (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Manuel Macias Gonzalez El objetivo principal de esta tesis ha sido la purificaci\u00f3n y caracterizaci\u00f3n del receptor [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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