{"id":38854,"date":"2018-03-09T09:38:38","date_gmt":"2018-03-09T09:38:38","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/manipulacion-genetica-del-hongo-fitopatogeno-botrytis-cinerrea-clonacion-del-gen-adha\/"},"modified":"2018-03-09T09:38:38","modified_gmt":"2018-03-09T09:38:38","slug":"manipulacion-genetica-del-hongo-fitopatogeno-botrytis-cinerrea-clonacion-del-gen-adha","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/manipulacion-genetica-del-hongo-fitopatogeno-botrytis-cinerrea-clonacion-del-gen-adha\/","title":{"rendered":"Manipulaci\u00f3n gen\u00e9tica del hongo fitopat\u00f3geno botrytis cinerrea: clonaci\u00f3n del gen adha."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Milagrosa Santos Hernandez <\/strong><\/h2>\n<p>Debido a la importancia de los da\u00f1os causados por botrytis cinerea, as\u00ed como a su amplia distribuci\u00f3n y elevado n\u00famero de cultivos que afecta, se han realizado numerosos estudios fisiol\u00f3gicos, bioqu\u00edmicos, epidemiol\u00f3gicos, gen\u00e9ticos, etc., Con el fin de conocer los distintos mecanismos que el hongo desarrolla durante el proceso infectivo. Muchas t\u00e9cnicas de biolog\u00eda molecular se han aplicado desde 1980 en este fitopat\u00f3geno, encaminadas en su gran mayor\u00eda, al estudio de enzimas que puedan intervenir en el ataque a su hu\u00e9sped tales como cutinasas, pectinasas, catalasas, o la producci\u00f3n de diversos metabolitos, o bien dedicados al estudio de la variabilidad gen\u00e9tica presente entre las distintas poblaciones. Sin embargo, y a pesar de todos estos trabajos no se conocen aspectos fundamentales del hongo como el control y los mecanismos de patogenicidad, la base molecular de las resistencias a fungicidas, etc.  el presente trabajo recoge un sistema de transformaci\u00f3n desarrollado en botrytis cinerea, mediante la utilizaci\u00f3n de dos tipos de vectores, uno de car\u00e1cter integrativo (put737) y otro de car\u00e1cter aut\u00f3nomo (pampf21). la transformaci\u00f3n de un hongo como botrytis es una herramienta poderosa que permite entender como se expresa el genoma f\u00fangico, y como controlan los genes, todas las actividades biol\u00f3gicas.  la utilizaci\u00f3n del vector put737 ha revelado la presencia del pl\u00e1smido libre en la c\u00e9lula receptora.La estabilizaci\u00f3n del pl\u00e1smido se produce como consecuencia de reordenaciones a trav\u00e9s de recombinaci\u00f3n in vivo.  la utilizaci\u00f3n de vector pampf21, el cual porta la regi\u00f3n ama1 que confiere capacidad de replicaci\u00f3n aut\u00f3noma el hongo tales como penicillium, aspergillus, pero no en otros como acremonium, confirma que dicha regi\u00f3n tambi\u00e9n permite la replicaci\u00f3n independiente en este hongo sin necesidad de ning\u00fan tipo de reorganizaci\u00f3n.  la segunda parte del trabajo recoge la clonaci\u00f3n de un gen implicad<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Manipulaci\u00f3n gen\u00e9tica del hongo fitopat\u00f3geno botrytis cinerrea: clonaci\u00f3n del gen adha.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Manipulaci\u00f3n gen\u00e9tica del hongo fitopat\u00f3geno botrytis cinerrea: clonaci\u00f3n del gen adha. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Milagrosa Santos Hernandez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 C\u00e1diz<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 23\/11\/1998<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jes\u00fas Manuel Cantoral Fern\u00e1ndez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: jose Mira gutierrez <\/li>\n<li>pedro Garc\u00eda Garc\u00eda (vocal)<\/li>\n<li>Fernando Leal sanchez (vocal)<\/li>\n<li>rosario Hern\u00e1ndez gal\u00e1n (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Milagrosa Santos Hernandez Debido a la importancia de los da\u00f1os causados por botrytis cinerea, as\u00ed como a 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