{"id":39349,"date":"1999-01-01T00:00:00","date_gmt":"1999-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/expresion-y-purificacion-del-receptor-hvdr-y-quimica-preliminar-para-el-estudio-de-su-zona-activa-por-marcaje-de-fotoafinidad\/"},"modified":"1999-01-01T00:00:00","modified_gmt":"1999-01-01T00:00:00","slug":"expresion-y-purificacion-del-receptor-hvdr-y-quimica-preliminar-para-el-estudio-de-su-zona-activa-por-marcaje-de-fotoafinidad","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/expresion-y-purificacion-del-receptor-hvdr-y-quimica-preliminar-para-el-estudio-de-su-zona-activa-por-marcaje-de-fotoafinidad\/","title":{"rendered":"Expresion y purificacion del receptor hvdr y quimica preliminar para el estudio  de su zona activa por marcaje de fotoafinidad."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Fernandez Gacio Ana Isabel <\/strong><\/h2>\n<p>En este trabajo de tesis se ha llevado a cabo la donaci\u00f3n del marco de lectura del receptor humano del calcitriol en un vector de expresi\u00f3n de e. Coli, creando el pl\u00e1smido pmw172-hvdr.  se ha expresado el receptor hvdr en la cepa de e. Coli bl21(de3), transformada con pmw172-hvdr, y se ha establecido un procedimiento de solubilizaci\u00f3n y purificaci\u00f3n del receptor as\u00ed expresado, que permite obtener el hvdr en cantidades de multimil\u00edgramos y con un grado de pureza del 85-90%.  se han dise\u00f1ado y sintetizado, v\u00eda ruta convergente de lythgoe, cuatro nuevos an\u00e1logos del calcitriol de cadena lateral semirr\u00edgida: (17z-1alfa-20-(4-(dimetilhidroxietil) fenil)-17,20-dideshidro-21,22,23,24,25,26,27- heptanor-vitamina d3, (17e)-1alfa-20(4-(dimetilhidroximetil) fenil)-17,20-dideshidro-21,22,23,24,25,26,27- heptanor-vitamina d3, (17z)-1alfa-20(3-(dimetilhidroximetil) fenil)-17,20-dideshidro-21,22,23,24,25,26,27- heptanor-vitamina d3, (17e)-1alfa-20-(3-(dimetilhidroximetil) fenil)-17,20-dideshidro-21,22,23,24,25,26,27- heptanor-vitamina d3.  se han sintetizado dos nuevos an\u00e1logos del calcitriol con una cadena lateral arom\u00e1tica con posibilidad de rotaci\u00f3n del enlace c-17(20): (17beta)- 1alfa-20-(4-(dimetilhidroximetil) fenil)-21,22,23,24,25,26,27-heptanor- vitamina d3,(17beta)-1alfa-20-(3-(dimetilhidroximetil)fenil) -21,22,23,24,25,26,27-heptanor-vitamina d3  se ha desarrollado la ruta de s\u00edntesis del primer ejemplo de un an\u00e1logo fotol\u00e1bil del calcitriol que incorpora un residuo diazir\u00ednico: (17z)- 1alfa-20-(3-((trifluorometil) -3h-1,2-diaziren-3-il)fenil)-17,20-dideshidro- 21,22,23,24,25,26,27-heptanor-vitamina d3. Este an\u00e1logo es de potencial inter\u00e9s para el marcaje de fotoafinidad del receptor hvdr.  se ha llevado a cabo un estudio preliminar de las conformaciones de las cadenas laterales de los an\u00e1logos semirr\u00edgidos sintetizados, con el objeto de establecer una correlaci\u00f3n entre la posici\u00f3n del hidroxilo de la cad<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Expresion y purificacion del receptor hvdr y quimica preliminar para el estudio  de su zona activa por marcaje de fotoafinidad.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Expresion y purificacion del receptor hvdr y quimica preliminar para el estudio  de su zona activa por marcaje de fotoafinidad. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Fernandez Gacio Ana Isabel <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Santiago de compostela<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1999<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Antonio Mouri\u00f1o Mosquera<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: rafael Suau suarez <\/li>\n<li>Manuel Freire rama (vocal)<\/li>\n<li>michael Breitenbach (vocal)<\/li>\n<li>claudio Palomo nicolau (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Fernandez Gacio Ana Isabel En este trabajo de tesis se ha llevado a cabo la donaci\u00f3n del [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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