{"id":41167,"date":"1999-01-01T00:00:00","date_gmt":"1999-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/transporte-nucleocitoplasmico-de-nucleoplasmina-y-rna-polimerasa-del-bacteriofago-t7-en-sistemas-in-vitro\/"},"modified":"1999-01-01T00:00:00","modified_gmt":"1999-01-01T00:00:00","slug":"transporte-nucleocitoplasmico-de-nucleoplasmina-y-rna-polimerasa-del-bacteriofago-t7-en-sistemas-in-vitro","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/transporte-nucleocitoplasmico-de-nucleoplasmina-y-rna-polimerasa-del-bacteriofago-t7-en-sistemas-in-vitro\/","title":{"rendered":"Transporte nucleocitoplasmico de nucleoplasmina y rna polimerasa del bacteriofago t7 en sistemas in vitro."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ricardo Andrade Pocino <\/strong><\/h2>\n<p>La comunicaci\u00f3n entre el n\u00facleo y el citoplasma es un proceso de gran importancia para la c\u00e9lula, ya que en el interior del n\u00facleo reside la informaci\u00f3n gen\u00e9tica, mientras que en el citoplasma se encuentra la maquinaria de s\u00edntesis proteica. Estas dos actividades, la nuclear y la citopl\u00e1smica, se encuentran separadas f\u00edsicamente por la envoltura nuclear, y para integrarlas es necesario que exista un mecanismo de transporte a trav\u00e9s de la envoltura nuclear.  el presente trabajo de investigaci\u00f3n profundiza en el conocimiento de las caracter\u00edsticas que rigen el transporte nucleocitopl\u00e1smico de prote\u00ednas cariof\u00edlicas. para ello hemos realizado dos ensayos de transporte in vitro.  el primero de ellos se basa en el estudio del transporte de la rna polimerasa del bacteri\u00f3fago t7, una prote\u00edna no cariof\u00edlica, a la cual se le ha a\u00f1adido una secuencia de localizaci\u00f3n nuclear que hace que se comporte como una prote\u00edna cariof\u00edlica en un ensayo de transporte nucleocitopl\u00e1smico en c\u00e9lulas nrk permeabilizadas con digitonina. En nuestro ensayo, el transporte de esta prote\u00edna recombinante cumple las siguientes caracter\u00edsticas: es dependiente de la secuencia de localizaci\u00f3n nuclear a\u00f1adida, es necesaria la acci\u00f3n de factores solubles, y el transporte es dependiente de energ\u00eda. adem\u00e1s, el hecho de que el anticuerpo monoclonal qe5, que reconoce las nucleoporinas nup214, nup153 y p62 bloquee el transporte de esta prote\u00edna, y no lo inhibian anticuerpos frente a la nup214 ni anticuerpos frente a la nup153 es un indicativo de que, en nuestras condiciones, la p62 est\u00e1 implicada en la importanci\u00f3n de esta prote\u00edna cariof\u00edlica.  de forma paralela, hemos desarrollado un nuevo ensayo de transporte in vitro cuyo resultado se detecta al microscopio electr\u00f3nico. Este m\u00e9todo consiste en colocar un n\u00facleo aislado de oocito de xenopus laevis en presencia de un extracto de huevos de xenopus en el interior de<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Transporte nucleocitoplasmico de nucleoplasmina y rna polimerasa del bacteriofago t7 en sistemas in vitro.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Transporte nucleocitoplasmico de nucleoplasmina y rna polimerasa del bacteriofago t7 en sistemas in vitro. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ricardo Andrade Pocino <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1999<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Miguel Ar\u00e9chaga Mart\u00ednez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: gregorio Garc\u00eda herdugo <\/li>\n<li>Manuel Jos\u00e9 Gayoso rodriguez (vocal)<\/li>\n<li>pl\u00e1cido Navas lloret (vocal)<\/li>\n<li>Jos\u00e9 Vilches troya (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ricardo Andrade Pocino La comunicaci\u00f3n entre el n\u00facleo y el citoplasma es un proceso de gran importancia [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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