{"id":42235,"date":"1999-01-01T00:00:00","date_gmt":"1999-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/la-transcriptasa-inversa-del-hiv-1-analisis-de-la-dimerizacion-y-de-la-inhibicion-por-terminadores-de-cadena\/"},"modified":"1999-01-01T00:00:00","modified_gmt":"1999-01-01T00:00:00","slug":"la-transcriptasa-inversa-del-hiv-1-analisis-de-la-dimerizacion-y-de-la-inhibicion-por-terminadores-de-cadena","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/la-transcriptasa-inversa-del-hiv-1-analisis-de-la-dimerizacion-y-de-la-inhibicion-por-terminadores-de-cadena\/","title":{"rendered":"La transcriptasa inversa del hiv-1: analisis de la dimerizacion y de la inhibicion por terminadores de cadena."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Cabodevilla Areta Jes\u00fas Felipe <\/strong><\/h2>\n<p>Se ha purificado mediante cromatograf\u00eda de afinidad por iones met\u00e1licos inmovilizados el polip\u00e9ptido de 66 kda, que forma parte de la transcriptasa inversa del hiv-1. El an\u00e1lisis de la muestra mediante cromatograf\u00eda de filtraci\u00f3n en gel indica que la prote\u00edna se encuentra en equilibrio entre la forma monom\u00e9rica (p66) y la dim\u00e9rica (p66\/p66). La actividad dna polimerasa rna dependiente reside exclusivamente en la forma homodim\u00e9rica de la enzima, aunque aproximadamente la mitad de los d\u00edmeros son inactivos. La dimerizaci\u00f3n de la subunidad p66 se produce en dos etapas. En la primera etapa las subunidades se asocian para formar un homod\u00edmero inactivo. Las sales anticaotr\u00f3picas y el aumento de temperatura favorecen la dimerizaci\u00f3n de la subunidad p66, aumentando tanto la velocidad a la que se alcanza el equilibrio como la estabilidad del homod\u00edmero. Se han estudiado los factores que afectan a la inhibici\u00f3n ejercida por los terminadores de cadena en los ensayos enzim\u00e1ticos utilizando el azt-tp como modelo. La incorporaci\u00f3n de un solo terminador de cadena en cualquier etapa durante la s\u00edntesis provocar\u00eda la terminaci\u00f3n de la polimerizaci\u00f3n e impedir\u00eda que se completara la s\u00edntesis viral. Hemos analizado la combinaci\u00f3n de terminadores de cadena utilizando un patr\u00f3n-cebador en condiciones en que \u00fanicamente existe un solo sitio de terminaci\u00f3n para cada an\u00e1logo. En esas condiciones, la combinaci\u00f3n de azt-tp y ddctp, dos inhibidores que compiten con distintos sustratos, inhiben sin\u00e9rgicamente la actividad enzim\u00e1tica. por el contrario la combinaci\u00f3n de azt-tp con ddttp, dos inhibidores competitivos mutuamente excluyentes, no muestran sinergia cuando se prueban sobre la rt purificada. Si se utiliza un patr\u00f3n-cebador que posee varios sitios potenciales de terminaci\u00f3n para azt-tp y ddctp, la sinergia entre ambos se pierde. Estos resultados son coherentes con el mecanismo de acci\u00f3n de los terminadores de cad<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>La transcriptasa inversa del hiv-1: analisis de la dimerizacion y de la inhibicion por terminadores de cadena.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 La transcriptasa inversa del hiv-1: analisis de la dimerizacion y de la inhibicion por terminadores de cadena. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Cabodevilla Areta Jes\u00fas Felipe <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1999<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Jos\u00e9 Mart\u00ednez Irujo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: esteban Santiago calvo <\/li>\n<li>laura Tarrago-litvak (vocal)<\/li>\n<li>simon Litvak (vocal)<\/li>\n<li>natalia Mar\u00eda Lopez moratalia (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Cabodevilla Areta Jes\u00fas Felipe Se ha purificado mediante cromatograf\u00eda de afinidad por iones met\u00e1licos inmovilizados el polip\u00e9ptido [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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