{"id":42833,"date":"2018-03-09T09:44:56","date_gmt":"2018-03-09T09:44:56","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/protocolo-de-crioconservacion-de-apices-en-lupulo\/"},"modified":"2018-03-09T09:44:56","modified_gmt":"2018-03-09T09:44:56","slug":"protocolo-de-crioconservacion-de-apices-en-lupulo","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/protocolo-de-crioconservacion-de-apices-en-lupulo\/","title":{"rendered":"Protocolo de crioconservaci\u00f3n de apices en lupulo"},"content":{"rendered":"<h2>Tesis doctoral de <strong> David Mart\u00ednez Fern\u00e1ndez <\/strong><\/h2>\n<p>Se ha desarrollado por primera vez en l\u00fapulo (humulus lupulus l.) Un protocolo de crioconservaci\u00f3n de \u00e1pices por el sistema de encapsulaci\u00f3n-deshidrataci\u00f3n en ocho variedades, con porcentaje de supervivencia entre 50 y 95% en funci\u00f3n del genotipo, despu\u00e9s de un precultivo en sacarosa 0,75 m, una deshidrataci\u00f3n en c\u00e1mara de flujo laminar de 4 h, congelaci\u00f3n por inmersi\u00f3n directa en nitr\u00f3geno l\u00edquido y descongelaci\u00f3n a temperatura ambiente.  igualmente, se describe un protocolo de crioconservaci\u00f3n para \u00e1pices de tallos de olivo (olea europaea l.), Por un m\u00e9todo de deshidrataci\u00f3n que permiti\u00f3 contrastar en ambas especies la relaci\u00f3n entre contenido en agua, formaci\u00f3n de cristales de hielo y porcentajes de supervivencia, aplicando para ello la t\u00e9cnica de calorimetr\u00eda diferencial de barrido. En \u00e1pices de l\u00fapulo se d\u00e1 una relaci\u00f3n entre ausencia de procesos de recristalizaci\u00f3n en muestras suficientemente deshidratadas y la formaci\u00f3n de transiciones t\u00e9rmicas de segundo orden a temperaturas entre -50\u00c2\u00ba y -60\u00c2\u00bac, lo cual justificar\u00eda la supervivencia de este material vegetal a temperaturas superiores al nitr\u00f3geno l\u00edquido (-80\u00c2\u00bac). Estos resultados sientan las bases que permitir\u00e1n crear la primera colecci\u00f3n de variedades de l\u00fapulo a temperaturas ultrabajas.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Protocolo de crioconservaci\u00f3n de apices en lupulo<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Protocolo de crioconservaci\u00f3n de apices en lupulo <\/li>\n<li><strong>Autor:<\/strong>\u00a0 David Mart\u00ednez Fern\u00e1ndez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Oviedo<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 30\/03\/1999<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Revilla Bahillo M. Angeles<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ricardo S\u00e1nchez tam\u00e9s <\/li>\n<li>rosa adela Arroyo Garc\u00eda (vocal)<\/li>\n<li>c\u00e9sar P\u00e9rez ruiz (vocal)<\/li>\n<li>Mar\u00eda Elena Gonz\u00e1lez benito (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de David Mart\u00ednez Fern\u00e1ndez Se ha desarrollado por primera vez en l\u00fapulo (humulus lupulus l.) Un protocolo de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[972,35,479,43479,8846,17,18],"tags":[16041,107361,105928,8894,8895,8893],"class_list":["post-42833","post","type-post","status-publish","format-standard","hentry","category-botanica","category-ciencias-de-la-vida","category-fisiologia-vegetal","category-metodos-termoanaliticos","category-oviedo","category-quimica","category-quimica-analitica","tag-cesar-perez-ruiz","tag-david-Martinez-fernandez","tag-maria-elena-gonzalez-benito","tag-revilla-bahillo-m-angeles","tag-ricardo-sanchez-tames","tag-rosa-adela-arroyo-garcia"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/42833","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=42833"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/42833\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=42833"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=42833"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=42833"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}