{"id":52821,"date":"2018-03-09T22:40:26","date_gmt":"2018-03-09T22:40:26","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estructura-de-la-exonucleasa-trex-1\/"},"modified":"2018-03-09T22:40:26","modified_gmt":"2018-03-09T22:40:26","slug":"estructura-de-la-exonucleasa-trex-1","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/barcelona\/estructura-de-la-exonucleasa-trex-1\/","title":{"rendered":"Estructura de la exonucleasa trex 1"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Marina Brucet Vinyals <\/strong><\/h2>\n<p>El objetivo general de la tesis realizada ha sido la caracterizaci\u00f3n de la prote\u00edna trex 1, una exonucleasa que parece desarrollar un papel importante en c\u00e9lulas de sistema inmunitario. Los estudios realizados con esta finalidad se pueden dividir en tres grandes bloques:     1,- determinaci\u00f3n de la estructura tridimensional de trex 1.     se ha determinado la estructura tridimensional de trex1. Se siguieron m\u00e9todos cristalizaci\u00f3n y difracci\u00f3n de rayos-x para llevar a cabo este objetivo. los resultados mostraron que esta prote\u00edna presenta una estructura homodim\u00e9rica que posiciona los centros activos de cada mon\u00f3mero en extremos opuestos de la cara frontal del d\u00edmero, y que la organizaci\u00f3n del centro activo de trex1 presenta elevada similitud con los centros activos de las exonucleasas de la familia deddh cuya estructura ha sido determinada. Adem\u00e1s, la estructura de trex1 ha sido determinada en presencia de un nucle\u00f3tido deoxitimidina monofosfato en cada uno de los centros activos, en dos condiciones distintas: en presencia de iones de litio, y en presencia de iones de magnesio. Los iones de litio inhiben la actividad de trex1 probablemente mediante la inhibici\u00f3n de la transferencia electr\u00f3nica necesaria para la reacci\u00f3n. Adem\u00e1s, provocan un ligero cambio de orientaci\u00f3n de algunos residuos del centro activo. El an\u00e1lisis de la estructura ha permitido tambi\u00e9n identificar un posible nuevo residuo catal\u00edtico no identificado hasta el momento en las exonucleasas de la familia deddh, la histidina 124. Finalmente, se ha identificado un motivo poliprolina en trex1 candidato a intervenir en la interacci\u00f3n con otras prote\u00ednas a trav\u00e9s de dominios sh3.     2,- interacci\u00f3n de trex1 con el dna.     el objetivo fue determinar las secuencias de dna para las que trex1 presenta mayor afinidad, seleccionando aquellas que mejor se un\u00edan a la prote\u00edna. Para ello, se utiliz\u00f3 la t\u00e9cnica de selecci\u00f3n de los sitios de uni\u00f3n. Se observ\u00f3 que trex1 prese<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estructura de la exonucleasa trex 1<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estructura de la exonucleasa trex 1 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Marina Brucet Vinyals <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 31\/05\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Antonio Celada Cotarelo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ignacio Fita rodriguez <\/li>\n<li>Andr\u00e9s Aguilera (vocal)<\/li>\n<li>Mar\u00eda Jes\u00fas Mac\u00edas hern\u00e1ndez (vocal)<\/li>\n<li>enrique Querol murillo (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Marina Brucet Vinyals El objetivo general de la tesis realizada ha sido la caracterizaci\u00f3n de la prote\u00edna [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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