{"id":52880,"date":"2006-02-06T00:00:00","date_gmt":"2006-02-06T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/desarrollo-de-protocolos-de-regeneracion-de-plantas-via-embriogenesis-somatica-y-de-transformacion-en-vid-vitis-vinifera-l\/"},"modified":"2006-02-06T00:00:00","modified_gmt":"2006-02-06T00:00:00","slug":"desarrollo-de-protocolos-de-regeneracion-de-plantas-via-embriogenesis-somatica-y-de-transformacion-en-vid-vitis-vinifera-l","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ingenieria-genetica\/desarrollo-de-protocolos-de-regeneracion-de-plantas-via-embriogenesis-somatica-y-de-transformacion-en-vid-vitis-vinifera-l\/","title":{"rendered":"Desarrollo de protocolos de regeneraci\u00f3n de plantas via embriogenesis somatica y de transformaci\u00f3n en vid (vitis vinifera l.)"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Lopez Perez Antonio  Jose <\/strong><\/h2>\n<p>En la memoria de investigaci\u00f3n presentadas se abordan dos objetivos principales:     1,- desarrollar un protocolo de regeneraci\u00f3n de plantas de vid por embriogenesis som\u00e1tica.     2,- establecer los par\u00e1metros b\u00e1sicos para la transformaci\u00f3n de c\u00e9lulas de callo embriog\u00e9nico mediante cocultivo con agrobacteium tumefaciens.     los resultados obtenidos en el primer objetivo han permitido establecer un protocolo de regeneraci\u00f3n de plantas de vid por embriog\u00e9nesis som\u00e1tica en tres variedades de uva de mesa (crimson seedless, sugraone y don mariano). el tipo de explanto (anteras u ovarios), la adici\u00f3n al medio de cultivo de carb\u00f3n activo y la morfolog\u00eda de los embriones som\u00e1ticos diferenciados a partir del callo embriog\u00e9nico son los tres factores que m\u00e1s han afectado a la eficiencia del protocolo de regeneraci\u00f3n.     una vez obtenido callo embriog\u00e9nico y establecido el protocolo de regeneraci\u00f3n, se abord\u00f3 la transformaci\u00f3n de c\u00e9lulas vegetales mediante agrobacterium en las variedades crimson seedless y sugraone. El establecimiento de un procedimiento de transformaci\u00f3n con una alta eficiencia se dividi\u00f3 en tres fases:     1,- establecimiento de la concentraci\u00f3n id\u00f3nea del antibi\u00f3tico kanamicina. los experimentos realizados mostraron que los callos embriog\u00e9nicos de crimson seedless y sugraone mostraron diferente sensibilidad a la kanamicina. Mientras que en la variedad crimson seedless se inhibi\u00f3 la diferenciaci\u00f3n de embriones som\u00e1ticos con 20 mg\/l de kanamicina, en la variedad sugraone fueron necesarios 50 mg\/l.     2,- influencia de la cepa de agrobacterium y la densidad de inoculo. los estudios de transformaci\u00f3n se abordaron con dos cepas de agrobacterium distintas (eha 105 y c58(pmp90)). as\u00ed mismo ambas cepas se utilizaron a distintas densidades de inoculo (do600 = 0,06 y 0,2). En todos los casos la cepa eha 105 mostr\u00f3 m\u00e1s eficiencia y se demostr\u00f3 que la densidad de inoculo m\u00e1s eficiente era diferente seg\u00fan la varie<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Desarrollo de protocolos de regeneraci\u00f3n de plantas via embriogenesis somatica y de transformaci\u00f3n en vid (vitis vinifera l.)<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Desarrollo de protocolos de regeneraci\u00f3n de plantas via embriogenesis somatica y de transformaci\u00f3n en vid (vitis vinifera l.) <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Lopez Perez Antonio  Jose <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 02\/06\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mercedes Dabauza Mico<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: angeles Pedre\u00f1o Mar\u00eda <\/li>\n<li>lorenzo Burgos (vocal)<\/li>\n<li>leandro Pe\u00f1a (vocal)<\/li>\n<li>ram\u00f3n Vidal jose (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Lopez Perez Antonio Jose En la memoria de investigaci\u00f3n presentadas se abordan dos objetivos principales: 1,- desarrollar [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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