{"id":53666,"date":"2018-03-09T22:41:23","date_gmt":"2018-03-09T22:41:23","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/rgt1-un-factor-transcripcional-sensible-a-la-glucosa-que-se-requiere-para-la-represion-del-gen-hxk2-de-saccharomyces-cerevisiae\/"},"modified":"2018-03-09T22:41:23","modified_gmt":"2018-03-09T22:41:23","slug":"rgt1-un-factor-transcripcional-sensible-a-la-glucosa-que-se-requiere-para-la-represion-del-gen-hxk2-de-saccharomyces-cerevisiae","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/radicales-libres\/rgt1-un-factor-transcripcional-sensible-a-la-glucosa-que-se-requiere-para-la-represion-del-gen-hxk2-de-saccharomyces-cerevisiae\/","title":{"rendered":"Rgt1, un factor transcripcional sensible a la glucosa, que se requiere para la represi\u00f3n del gen hxk2 de saccharomyces cerevisiae"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Carlos Aaron Palomino Martinez <\/strong><\/h2>\n<p>Durante los cuatro a\u00f1os de beca ficyt he trabajado sobre la prote\u00edna rgt1, un factor transcripcional que reprime la expresi\u00f3n de determinados genes en ausencia de glucosa, y que participa en la expresi\u00f3n de otros en presencia de la misma. Mediante ensayos northern, rt-pcr y an\u00e1lisis de la expresi\u00f3n beta-galactosidasa hemos demostrado que rgt1 se expresa de forma constitutiva. Adem\u00e1s gracias a experimentos de localizaci\u00f3n utilizando fusiones con gfp hemos verificado que se localiza constitutivamente en el n\u00facleo. Analizando la expresi\u00f3n del promotor hxk2 fusionado en pauta de lectura al gen indicador de beta-galactosidasa en mutantes rgt1 (obtenidos mediante interrupci\u00f3n gen\u00f3mica) y distintas fuentes de carbono hemos demostrado que rgt1 regula la expresi\u00f3n del gen hxk2 en s.Cerevisiae, inhibi\u00e9ndola en medios con fuentes de carbono no fermentables. Sin embargo un mutante rgt1 no tiene alterada la expresi\u00f3n del gen suc2 (inhibida por un complejo proteico del cual hxk2 y mig1 forman parte), que codifica para la invertasa. Estos datos son coherentes con otros resultados obtenidos que demuestran que mig y hxk2 no tienen alterado su patr\u00f3n de localizaci\u00f3n celular en mutantes rgt1. para saber c\u00f3mo act\u00faa rgt1 sobre el promotor hkk2, se realizaron ensayos de retardo de la movilidad electrofor\u00e9tica utilizando como sondas las secuencias reguladoras del promotor del gen hxk2 marcadas radiactivamente. Existen diferentes complejos adn-proteina dependiendo de la fuente de carbono. Para demostrar que rgt1 se une directamente al promotor hxk2 se repitieron estos ensayos con una prote\u00edna rgt1 recombinante purificada, y se realizaron ensayos de inmunoprecipitaci\u00f3n de la cromatina (cglp). se han realizado ensayos de doble h\u00edbrido, coinmunopreciptaci\u00f3n y coprecipitaci\u00f3n gst -pull-down para demostrar la interacci\u00f3n in vivo e in vitro entre rgt1 y las prote\u00ednas hxk2 y med8 en distintas condiciones de crecimiento. Adem\u00e1s hemos acotado la regi\u00f3n concreta de hxk<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Rgt1, un factor transcripcional sensible a la glucosa, que se requiere para la represi\u00f3n del gen hxk2 de saccharomyces cerevisiae<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Rgt1, un factor transcripcional sensible a la glucosa, que se requiere para la represi\u00f3n del gen hxk2 de saccharomyces cerevisiae <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Carlos Aaron Palomino Martinez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Oviedo<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 30\/06\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Pilar Herrero Espilez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: pedro Sanchez lazo <\/li>\n<li>esperanza Cerdan (vocal)<\/li>\n<li>Luis Rodriguez dominguez (vocal)<\/li>\n<li> Prieto alaman Jos\u00e9 Antonio (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carlos Aaron Palomino Martinez Durante los cuatro a\u00f1os de beca ficyt he trabajado sobre la prote\u00edna rgt1, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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