{"id":54934,"date":"2006-06-10T00:00:00","date_gmt":"2006-06-10T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/abordajes-proteomicos-para-el-estudio-de-la-secrecion-no-clasica-y-la-fosforilacion-en-saccharomyces-cerevisiae\/"},"modified":"2006-06-10T00:00:00","modified_gmt":"2006-06-10T00:00:00","slug":"abordajes-proteomicos-para-el-estudio-de-la-secrecion-no-clasica-y-la-fosforilacion-en-saccharomyces-cerevisiae","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/microbiologia\/abordajes-proteomicos-para-el-estudio-de-la-secrecion-no-clasica-y-la-fosforilacion-en-saccharomyces-cerevisiae\/","title":{"rendered":"Abordajes prote\u00f3micos para el estudio de la secreci\u00f3n no cl\u00e1sica y la fosforilaci\u00f3n en saccharomyces cerevisiae"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Elena L\u00f3pez Villar <\/strong><\/h2>\n<p>El trabajo realizado en colaboraci\u00f3n entre el departamento de microbiolog\u00eda ii de la universidad complutense de Madrid (espa\u00f1a) y el departamento de bioqu\u00edmica y biolog\u00eda molecular de la universidad de odense (dinamarca), ha permitido desarrollar estudios de secreci\u00f3n prot\u00e9ica y fosforilaci\u00f3n en saccharomyces cerevisiae.     un elevado n\u00famero de prote\u00ednas, cl\u00e1sicamente descritas como citos\u00f3licas, al carecer de p\u00e9ptido se\u00f1al para su entrada en la ruta de secreci\u00f3n, han sido localizadas en el exterior de distintos microorganismos, relacion\u00e1ndose con rutas de exportaci\u00f3n no convencional. La combinaci\u00f3n de aproximaciones de biolog\u00eda molecular, microscop\u00eda de fluorescencia y t\u00e9cnicas prote\u00f3micas, ha permitido profundizar en la localizaci\u00f3n subcelular de una de estas prote\u00ednas, eno2p, y su relaci\u00f3n con rutas de secreci\u00f3n no cl\u00e1sicas. Se comprob\u00f3 que los 169 amino\u00e1cidos aminoterminales son suficientes para exportar invertasas intracelular al espacio peripl\u00e1smico. M\u00e1s a\u00fan, estudios de microscopia confocal indicaron que dicho fragmento, fusionado a la prote\u00edna verde fluorescente, se localizaba no solo en el citoplasma sino tambi\u00e9n el membrana plasm\u00e1tica. Adem\u00e1s, mediante estudios  prote\u00f3micos consistentes en fraccionamiento e identificaci\u00f3n de prote\u00ednas de membranas de s.Cerevisiae, se detect\u00f3 eno2p, como una prote\u00edna asociada a membranas.     por otro lado, se ha puesto a punto una estrategia para la identificaci\u00f3n y cuantificaci\u00f3n de la fosforilaci\u00f3n de prote\u00ednas en levaduras tras su estimulaci\u00f3n con factor alfa. El marcaje metab\u00f3lico mediante silac, de c\u00e9lulas con y sin tratamiento, permito la cuantificaci\u00f3n relativa de las prote\u00ednas y fosfop\u00e9ptidos. Para el enriquecimiento en fosfop\u00e9ptidos se han combinado distintos tipos de cromatograf\u00edas (atp sepharoese, imac (quiagen) y di\u00f3xido de titanio (tio2) con dhb), de las cuales la combinaci\u00f3n imac (quiagen) junto con tio2\/dhb mostr\u00f3 el mejor rendimiento. Mediante la u<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Abordajes prote\u00f3micos para el estudio de la secreci\u00f3n no cl\u00e1sica y la fosforilaci\u00f3n en saccharomyces cerevisiae<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Abordajes prote\u00f3micos para el estudio de la secreci\u00f3n no cl\u00e1sica y la fosforilaci\u00f3n en saccharomyces cerevisiae <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Elena L\u00f3pez Villar <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 06\/10\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Concepcion Gil Garcia<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: cesar Nombella cano <\/li>\n<li>Mar\u00eda no Esteban rodriguez (vocal)<\/li>\n<li>Juan  pablo Albar ramirez (vocal)<\/li>\n<li>amparo Querol sim\u00f3n (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Elena L\u00f3pez Villar El trabajo realizado en colaboraci\u00f3n entre el departamento de microbiolog\u00eda ii de la universidad [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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