{"id":55655,"date":"2018-03-09T22:43:23","date_gmt":"2018-03-09T22:43:23","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/purificacion-y-clonacion-de-sistemas-enzimaticos-para-la-bioconversion-de-monofenoles-en-difenoles-polifenol-oxidasa-tirosinasa-y-fenol-hidroxilasa\/"},"modified":"2018-03-09T22:43:23","modified_gmt":"2018-03-09T22:43:23","slug":"purificacion-y-clonacion-de-sistemas-enzimaticos-para-la-bioconversion-de-monofenoles-en-difenoles-polifenol-oxidasa-tirosinasa-y-fenol-hidroxilasa","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/purificacion-y-clonacion-de-sistemas-enzimaticos-para-la-bioconversion-de-monofenoles-en-difenoles-polifenol-oxidasa-tirosinasa-y-fenol-hidroxilasa\/","title":{"rendered":"Purificaci\u00f3n y clonaci\u00f3n de sistemas enzim\u00e1ticos para la bioconversi\u00f3n de monofenoles en difenoles: polifenol oxidasa, tirosinasa y fenol hidroxilasa"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Esteban Orenes Pi\u00f1ero <\/strong><\/h2>\n<p>Los difenoles son compuestos con alto valor comercial por su capacidad antioxidante que se obtienen a partir de la hidroxilaci\u00f3n de monofenoles. Dos enzimas se encargan de esta bioconversi\u00f3n, tirosinasa, tambi\u00e9n llamada polifenol oxidasa en plantas, y fenol hidroxilasa. Hasta el momento, no se han descrito procesos biocatal\u00edticos eficientes para la obtenci\u00f3n de difenoles. Por tanto, el objetivo de este proyecto de tesis es la obtenci\u00f3n, mediante purificaci\u00f3n y clonaci\u00f3n, de polifenol oxidasa\/tirosinasa y fenol hidroxilasa de tres fuentes separadas en la escala filogen\u00e9tica y el estudio de la capacidad de bios\u00edntesis de estas enzimas ya sea mediante inmovilizaci\u00f3n enzim\u00e1tica en diferentes soportes o mediante un sistema de bioconversi\u00f3n de c\u00e9lulas transformadas completas.  Los substratos utilizados, fenol y tirosol, tienen gran importancia comercial. El primero es un compuesto altamente contaminante en refiner\u00edas petrol\u00edferas y plantas petroqu\u00edmicas, y el segundo, da lugar tras su hidroxilaci\u00f3n al hidroxitirosol, un nutrac\u00e9utico de alto valor a\u00f1adido con gran capacidad antioxidante y que estabiliza al aceite de oliva. El sistema de c\u00e9lulas transformadas completas permiti\u00f3  bioconversiones de los monofenoles en sus correspondientes difenoles del 100% por vez primera en la bibliograf\u00eda.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Purificaci\u00f3n y clonaci\u00f3n de sistemas enzim\u00e1ticos para la bioconversi\u00f3n de monofenoles en difenoles: polifenol oxidasa, tirosinasa y fenol hidroxilasa<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Purificaci\u00f3n y clonaci\u00f3n de sistemas enzim\u00e1ticos para la bioconversi\u00f3n de monofenoles en difenoles: polifenol oxidasa, tirosinasa y fenol hidroxilasa <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Esteban Orenes Pi\u00f1ero <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 24\/11\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Alvaro Sanchez Ferrer<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal:  L\u00f3pez roca Jos\u00e9 Mar\u00eda <\/li>\n<li>josefa Escribano cebrian (vocal)<\/li>\n<li>felix ramon Romojaro almela (vocal)<\/li>\n<li>manuela Perez gilabert (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Esteban Orenes Pi\u00f1ero Los difenoles son compuestos con alto valor comercial por su capacidad antioxidante que se [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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