{"id":56105,"date":"2018-03-09T22:43:47","date_gmt":"2018-03-09T22:43:47","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estructura-organizacion-y-dinamica-del-aparato-de-golgi-a-15aoc-papel-de-los-tubulos-en-el-transporte-intra-golgi\/"},"modified":"2018-03-09T22:43:47","modified_gmt":"2018-03-09T22:43:47","slug":"estructura-organizacion-y-dinamica-del-aparato-de-golgi-a-15aoc-papel-de-los-tubulos-en-el-transporte-intra-golgi","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/estructura-organizacion-y-dinamica-del-aparato-de-golgi-a-15aoc-papel-de-los-tubulos-en-el-transporte-intra-golgi\/","title":{"rendered":"Estructura, organizaci\u00f3n y din\u00e1mica del aparato de golgi a 15\u00c2\u00bac: papel de los t\u00fabulos en el transporte intra-golgi"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Emma Mart\u00ednez Alonso <\/strong><\/h2>\n<p>En la presente tesis doctoral hemos estudiado la estructura, organizaci\u00f3n y din\u00e1mica del complejo de golgi en c\u00e9lulas hela incubadas a 15\u00c2\u00bac, condiciones en las que se induce el bloqueo del tr\u00e1fico a nivel del compartimento intermedio. Para ello hemos utilizado diferentes aproximaciones t\u00e9cnicas como son la microscop\u00eda electr\u00f3nica, crioinmunocitoqu\u00edmica ultraestructural, t\u00e9cnicas de inmunofluorescencia y microscop\u00eda confocal in vivo.    el an\u00e1lisis de la distribuci\u00f3n de los marcadores del compartimento intermedio  (ergic-53 y kdelr) confirma un patr\u00f3n de marcaje m\u00e1s concentrado en condiciones de 15\u00c2\u00bac que en condiciones fisiol\u00f3gicas en c\u00e9lulas hela, resultados descritos anteriormente por otros autores en otros tipos celulares. Sin embargo, la tinci\u00f3n con diferentes enzimas de glicosilaci\u00f3n residentes del aparato de golgi, como son manosidasa ii (manii), galactosiltransferasa (galtr) y n-acetil-galactosaminiltransferasa (galnactr) han puesto en evidencia la formaci\u00f3n de t\u00fabulos derivados del aparato de golgi en estas condiciones de baja temperatura. Por otro lado, la tinci\u00f3n con las prote\u00ednas de matriz del aparato de golgi, gm130, giantina y golgina-84 no marcan los t\u00fabulos que contienen las enzimas. El marcaje contra la prote\u00edna tgn-46, marcador del tgn, demuestra que estos t\u00fabulos no son derivados del tgn ya que no son positivos para la prote\u00edna. El an\u00e1lisis funcional con el mutante sensible a temperatura del virus de la estomatitis vesicular (vsv-g) y el fragmento b de la toxina shiga, ha demostrado que los t\u00fabulos derivados del golgi a 15\u00c2\u00bac no contienen ning\u00fan tipo de carga ni anter\u00f3grado ni retr\u00f3grado, respectivamente. De la caracterizaci\u00f3n de la formaci\u00f3n de los t\u00fabulos derivados del golgi con diferentes drogas y reactivos, podemos decir que la formaci\u00f3n de los t\u00fabulos es dependiente de energ\u00eda y de un citoesqueleto intacto de actina y microt\u00fabulos. Por el contrario, la acci\u00f3n de la brefeldina a o cicloheximida no tiene<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estructura, organizaci\u00f3n y din\u00e1mica del aparato de golgi a 15\u00c2\u00bac: papel de los t\u00fabulos en el transporte intra-golgi<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estructura, organizaci\u00f3n y din\u00e1mica del aparato de golgi a 15\u00c2\u00bac: papel de los t\u00fabulos en el transporte intra-golgi <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Emma Mart\u00ednez Alonso <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 15\/12\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Angel Martinez Menarguez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jaime Renau piqueras <\/li>\n<li>adelina Zuasti elizondo (vocal)<\/li>\n<li> Garc\u00eda borr\u00f3n Jos\u00e9 Carlos (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Emma Mart\u00ednez Alonso En la presente tesis doctoral hemos estudiado la estructura, organizaci\u00f3n y din\u00e1mica del complejo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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