{"id":56161,"date":"2018-03-09T22:43:51","date_gmt":"2018-03-09T22:43:51","guid":{"rendered":""},"modified":"2018-03-09T22:43:51","modified_gmt":"2018-03-09T22:43:51","slug":"caracterizacion-inmunoqua%c2%admica-y-genetica-de-la-produccion-de-las-enterocinas-l50p-y-q-por-enterococcus-faecium-l50-secuencia-nucleota%c2%addica-y-analisis-funcional-de-los-plasmidos-pciz1-y-pc","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-inmunoqua%c2%admica-y-genetica-de-la-produccion-de-las-enterocinas-l50p-y-q-por-enterococcus-faecium-l50-secuencia-nucleota%c2%addica-y-analisis-funcional-de-los-plasmidos-pciz1-y-pc\/","title":{"rendered":"Caracterizaci\u00f3n inmunoqu\u00edmica y gen\u00e9tica de la producci\u00f3n de las enterocinas l50,p y q por enterococcus faecium l50. secuencia nucleot\u00eddica y an\u00e1lisis funcional de los pl\u00e1smidos pciz1 y pciz2"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Raquel Criado Garc\u00eda <\/strong><\/h2>\n<p>Durante esta tesis doctoral se han obtenido anticuerpos policlonales de especificidad predeterminada frente a la enterocina l50 (entl50, sistema de dos p\u00e9ptidos [entl50a y entl50b]) y enterocina q (entq), los cuales han sido empleados junto con anticuerpos frente a la enterocina p (entp), obtenidos previamente, en diversos ensayos inmunoenzim\u00e1ticos (elisa), lo que ha permitido la detecci\u00f3n espec\u00edfica y la cuantificaci\u00f3n de las enterocinas producidas por e. Faecium l50. Adem\u00e1s, la combinaci\u00f3n de ensayos inmunoenzim\u00e1ticos y microbiol\u00f3gicos ha permitido determinar el efecto de la temperatura en la producci\u00f3n de estas bacteriocinas. A este respecto, la m\u00e1xima producci\u00f3n de entl50 (entl50a y entl50b), entp y entq se detect\u00f3 en cultivos desarrollados a 25, 37 y 47\u00c2\u00bac, respectivamente.  el trabajo de investigaci\u00f3n desarrollado continu\u00f3 con la caracterizaci\u00f3n gen\u00e9tica de la producci\u00f3n de enterocinas por e. Faecium l50. Para ello, se procedi\u00f3 a la localizaci\u00f3n de los determinantes gen\u00e9ticos asociados a los fenotipos de producci\u00f3n e inmunidad de entl50, entq y entp mediante experiencias de hibridaci\u00f3n adn-adn (southern-blot) y curaci\u00f3n plasm\u00eddica, lo que demostr\u00f3 que se encuentran ligados gen\u00e9ticamente y se localizan en los pl\u00e1smidos pciz1 (aprox. 50 kb) y pciz2 (aprox. 7,4 kb) y en el cromosoma, respectivamente. A continuaci\u00f3n, se inici\u00f3 la caracterizaci\u00f3n molecular de pciz1 y pciz2 mediante la determinaci\u00f3n de su secuencia nucleot\u00eddica parcial y total, respectivamente. El an\u00e1lisis inform\u00e1tico de las secuencias nucleot\u00eddicas obtenidas permiti\u00f3 identificar los hipot\u00e9ticos genes presentes en estos pl\u00e1smidos, entre los que se incluyen los que constituyen los operones y\/o agrupaciones g\u00e9nicas implicados en la producci\u00f3n, inmunidad y\/o secreci\u00f3n de entl50, entq y entp. En lo que respecta al pl\u00e1smido pciz1, \u00e9ste incluye 27 hipot\u00e9ticos genes agrupados en cuatro regiones implicadas presumiblemente en la: (i) producci\u00f3n de entp (orf1-3)<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n inmunoqu\u00edmica y gen\u00e9tica de la producci\u00f3n de las enterocinas l50,p y q por enterococcus faecium l50. secuencia nucleot\u00eddica y an\u00e1lisis funcional de los pl\u00e1smidos pciz1 y pciz2<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n inmunoqu\u00edmica y gen\u00e9tica de la producci\u00f3n de las enterocinas l50,p y q por enterococcus faecium l50. secuencia nucleot\u00eddica y an\u00e1lisis funcional de los pl\u00e1smidos pciz1 y pciz2 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Raquel Criado Garc\u00eda <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 15\/12\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Luis Miguel Cintas Izarra<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda Del Rosario Mart\u00edn De Santos <\/li>\n<li>Victoria Francia M\u00aa (vocal)<\/li>\n<li>Rosa Mar\u00eda Del Campo  Moreno (vocal)<\/li>\n<li>F. Nes Ingolf (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Raquel Criado Garc\u00eda Durante esta tesis doctoral se han obtenido anticuerpos policlonales de especificidad predeterminada frente a [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[124057,92493,6115,124055,52567,124056],"class_list":["post-56161","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-f-nes-ingolf","tag-luis-miguel-cintas-izarra","tag-maria-del-rosario-martin-de-santos","tag-raquel-criado-garcia","tag-rosa-maria-del-campo-moreno","tag-victoria-francia-ma"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/56161","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=56161"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/56161\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=56161"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=56161"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=56161"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}