{"id":56361,"date":"2018-03-09T22:44:04","date_gmt":"2018-03-09T22:44:04","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/insight-into-the-evolution-of-anaphylatoxin-receptors-through-the-study-of-c3a-and-c5a-receptors-in-rainbow-trout-oncorhynchus-mykiss\/"},"modified":"2018-03-09T22:44:04","modified_gmt":"2018-03-09T22:44:04","slug":"insight-into-the-evolution-of-anaphylatoxin-receptors-through-the-study-of-c3a-and-c5a-receptors-in-rainbow-trout-oncorhynchus-mykiss","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/tecnologia-bioquimica\/insight-into-the-evolution-of-anaphylatoxin-receptors-through-the-study-of-c3a-and-c5a-receptors-in-rainbow-trout-oncorhynchus-mykiss\/","title":{"rendered":"Insight into the evolution of anaphylatoxin receptors through the study of c3a and c5a receptors in rainbow trout (oncorhynchus mykiss)"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Hani Youssef Boshra <\/strong><\/h2>\n<p>Activation of the complement system results in the generation of anaphylatoxins c3a, c4a and c5a. In mammals, it has been shown that c3a and c5a elicit biological responses through their interactions with c3 and c5 receptors respectively. Although both receptors share a high degree of homology, until recently, very little was understood about the evolution of these receptors. In particular, it was not known whether or not these receptors were represented by a single common ancestral molecule in lower vertebrates. Here, we present the clonning and functional characterization of bona fise c3ar and c5ar in rainbow trout. While both receptors share a high degree of homology with their mammalian counterparts, the secondary structure of c3ar is unique in its truncated second extracellular loop. Antibodies generated against c3ar inhibited trout c3a-mediated intracellular calcium mobilization in trout leukocytes. While recombinantly expressed c5a was able to induce chemotaxis in trout peripheral leukocytes, antibodies generated against c5ar were found to inhibit this activity. Both c3ar and c5ar are expressed primary on b-cells, and to a lesser extent, on granulocytes. Our resluts indicate that, although highly homologous, the divergence of these two molecules into distinct receptors occurred prior to the emergence of teleost fish.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Insight into the evolution of anaphylatoxin receptors through the study of c3a and c5a receptors in rainbow trout (oncorhynchus mykiss)<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Insight into the evolution of anaphylatoxin receptors through the study of c3a and c5a receptors in rainbow trout (oncorhynchus mykiss) <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Hani Youssef Boshra <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 20\/12\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Oriol Su\u00f1er Capdevila<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: carmen Amaro gonz\u00e1lez <\/li>\n<li>ariadna Sitja bobadilla (vocal)<\/li>\n<li>merc\u00e9 Mart\u00ed ripoll (vocal)<\/li>\n<li>m. rosa Sarrias forn\u00e9s (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Hani Youssef Boshra Activation of the complement system results in the generation of anaphylatoxins c3a, c4a and [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36945,20868,2599,1714],"tags":[124515,21435,124514,29480,37135,56881],"class_list":["post-56361","post","type-post","status-publish","format-standard","hentry","category-ictiologia","category-inmunologia-veterinaria","category-piscicultura","category-tecnologia-bioquimica","tag-ariadna-sitja-bobadilla","tag-carmen-amaro-gonzalez","tag-hani-youssef-boshra","tag-jose-oriol-suner-capdevila","tag-m-rosa-sarrias-fornes","tag-merce-marti-ripoll"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/56361","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=56361"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/56361\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=56361"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=56361"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=56361"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}