{"id":56773,"date":"2018-03-09T22:44:33","date_gmt":"2018-03-09T22:44:33","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-de-una-protea%c2%adna-nativa-y-recombinante-de-giardia-lamblia-capaz-de-ligar-acidos-grasos\/"},"modified":"2018-03-09T22:44:33","modified_gmt":"2018-03-09T22:44:33","slug":"aislamiento-de-una-protea%c2%adna-nativa-y-recombinante-de-giardia-lamblia-capaz-de-ligar-acidos-grasos","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/parasitologia-animal\/aislamiento-de-una-protea%c2%adna-nativa-y-recombinante-de-giardia-lamblia-capaz-de-ligar-acidos-grasos\/","title":{"rendered":"Aislamiento de una prote\u00edna nativa y recombinante de giardia lamblia capaz de ligar \u00e1cidos grasos."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  D\u00edaz De La Guardia Quiles Rafael <\/strong><\/h2>\n<p>Giardia es un protozoo flagelado que infecta el tracto superior del intestino delgado de muchas especies de vertebrados, entre las que se encuentra el hombre, siendo la causa m\u00e1s frecuente de infecci\u00f3n gastrointestinal humana producida por protozoos par\u00e1sitos.  en este trabajo, hemos caracterizado algunas de las propiedades que presenta una prote\u00edna de giardia lamblia, aislada en nuestro laboratorio, que posee caracter\u00edsticas similares a una fabp, tales como:  * su peso molecular exacto.  * su capacidad para ligar \u00e1cidos grasos, junto con la cin\u00e9tica de desplazamiento de estos y la constante de desplazamiento.  * el rendimiento en su purificaci\u00f3n.  * la capacidad que tiene de formar agregados proteicos, junto con la p\u00e9rdida de funcionalidad cuando ocurre esto y su posible significado biol\u00f3gico.  * la proporci\u00f3n de amino\u00e1cidos que componen un fragmento de esta prote\u00edna, poseyendo el extremo n-terminal bloqueado.   tambi\u00e9n hemos conocido la secuencia de nucle\u00f3tidos que la codifica, mediante la realizaci\u00f3n de diversos acercamientos gen\u00e9ticos tales como:  * la amplificaci\u00f3n del gen que codifica para esta prote\u00edna mediante la utilizaci\u00f3n de dna gen\u00f3mico, y dos cebadores dise\u00f1ados de una secuencia consenso de nucle\u00f3tidos de la secuencia de esta misma prote\u00edna pero de otros organismos.  * la realizaci\u00f3n de una reacci\u00f3n 3 prima race a partir de mrna y un cebador espec\u00edfico de la secuencia consenso junto con dos inespec\u00edficos creados de modo artificial.  * la realizaci\u00f3n de una genoteca de expresi\u00f3n, la cual hemos rastreado, obtenido un clon positivo, cuya secuencia ha sido analizada, encontrando adem\u00e1s en su interior un intr\u00f3n, provocando que se discuta la idea actualmente aceptada de que los intrones entraron en el genoma de los eucariotas a trav\u00e9s de las mitocondrias.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aislamiento de una prote\u00edna nativa y recombinante de giardia lamblia capaz de ligar \u00e1cidos grasos.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aislamiento de una prote\u00edna nativa y recombinante de giardia lamblia capaz de ligar \u00e1cidos grasos. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  D\u00edaz De La Guardia Quiles Rafael <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Granada<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/01\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Antonio Osuna Carrillo De Albornoz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Mar\u00eda Medina jimenez <\/li>\n<li>Jorge  Antonio Guisantes del barco (vocal)<\/li>\n<li> Rodrigues de sousa Mar\u00eda do ceu (vocal)<\/li>\n<li>anne Francoise petavy (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de D\u00edaz De La Guardia Quiles Rafael Giardia es un protozoo flagelado que infecta el tracto superior del [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[2119,661],"tags":[125455,3524,125453,17954,7301,125454],"class_list":["post-56773","post","type-post","status-publish","format-standard","hentry","category-genetica-animal","category-parasitologia-animal","tag-anne-francoise-petavy","tag-antonio-osuna-carrillo-de-albornoz","tag-diaz-de-la-guardia-quiles-rafael","tag-jorge-antonio-guisantes-del-barco","tag-jose-maria-medina-jimenez","tag-rodrigues-de-sousa-maria-do-ceu"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/56773","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=56773"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/56773\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=56773"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=56773"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=56773"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}