{"id":56895,"date":"2007-05-02T00:00:00","date_gmt":"2007-05-02T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/substrats-fluoroga%c2%a8nics-de-ceramidases-i-esfingosina-1-fosfat-liasa-disseny-sa%c2%adntesi-i-aplicacio-en-la-recerca-de-nous-inhibidors-enzima-tics\/"},"modified":"2007-05-02T00:00:00","modified_gmt":"2007-05-02T00:00:00","slug":"substrats-fluoroga%c2%a8nics-de-ceramidases-i-esfingosina-1-fosfat-liasa-disseny-sa%c2%adntesi-i-aplicacio-en-la-recerca-de-nous-inhibidors-enzima-tics","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica-organica\/substrats-fluoroga%c2%a8nics-de-ceramidases-i-esfingosina-1-fosfat-liasa-disseny-sa%c2%adntesi-i-aplicacio-en-la-recerca-de-nous-inhibidors-enzima-tics\/","title":{"rendered":"Substrats fluorog\u00e9nics de ceramidases i esfingosina-1-fosfat liasa. disseny, s\u00edntesi i aplicaci\u00f3 en la recerca de nous inhibidors enzim\u00c1\u00a0tics"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Carmen Bedia Girb\u00e9s <\/strong><\/h2>\n<p>Los esfingol\u00edpidos son una familia lip\u00eddica de compuestos de gran importancia estructural y funcional en la c\u00e9lula. La ceramida, el esfingol\u00edpido central en el metabolismo del resto de esfingol\u00edpidos, es un segundo mensajero celular y su funci\u00f3n principal es la inducci\u00f3n de la apoptosis. La degradaci\u00f3n de la ceramida a trav\u00e9s de ceramidasas y esfingosina quinasa, da lugar a otro esfingol\u00edpido de gran importancia, la esfingosina-1-fosfato, que tiene propiedades prolfierativas y mitog\u00e9ncias, siendo \u00e9stas totalmente opuestas a la funci\u00f3n de la ceramida. la esfingosina-1-fosfato puede ser a su vez degradada por el enzima esfingosina-1-fosfato liasa. por todo ello, las ceramidasas y la esfingosina-1-fosfato liasa son enzimas de gran importancia ya que regulan el re\u00f3stato ceramida\/esfingosina-1-fosfato, que puede determinar la supervivencia de la c\u00e9lula. Para poder determinar la actividad de estos enzimas y disponer de un m\u00e9todo eficaz para hallar nuevos inhibidores enzim\u00e1ticos entre librer\u00edas de an\u00e1logos de esfingol\u00edpidos, se dise\u00f1aron y sintetizaron unos sustratos fluorog\u00e9ncios. estos sustratos se idearon para que despu\u00e9s de sufrir la acci\u00f3n enzim\u00e1tica, dieran lugar a un aldeh\u00eddo inestable que al sufrir beta-eliminaci\u00f3n liberara umbeliferona, la cual es fluorescente.  siguiendo esta idea y los requisitos estructurales de estos enzimas para sus sustratos, se dise\u00f1aron y sintetizaron los dos sustratos fluorog\u00e9nicos. A partir de estos, se desarroll\u00f3 una metodolog\u00eda para la criba masiva de librer\u00edas de mol\u00e9culas sintetizadas en nuestro grupo, como potenciales inhibidores enzim\u00e1ticos. Estos m\u00e9todos, que ten\u00edan lugar en placas de 96 pocillos, primero se desarrollaron in vitro, utilizando como fuente enzim\u00e1tica homogenados de h\u00edgado de rata.  posteriormente, se aplicaron estos ensayos a cultivos de c\u00e9lulas. En el caso de ceramidasas, se consigui\u00f3 un m\u00e9todo espec\u00edfico para determinar la actividad de ceramidasa \u00e1cida utilizando c\u00e9<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Substrats fluorog\u00e9nics de ceramidases i esfingosina-1-fosfat liasa. disseny, s\u00edntesi i aplicaci\u00f3 en la recerca de nous inhibidors enzim\u00c1\u00a0tics<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Substrats fluorog\u00e9nics de ceramidases i esfingosina-1-fosfat liasa. disseny, s\u00edntesi i aplicaci\u00f3 en la recerca de nous inhibidors enzim\u00c1\u00a0tics <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Carmen Bedia Girb\u00e9s <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 05\/02\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Gemma Fabri\u00c1\u00a0s Domingo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: albert Moyano baldoire <\/li>\n<li>thierry Levade (vocal)<\/li>\n<li>Antonio Gomez mu\u00f1oz (vocal)<\/li>\n<li>isabel Haro villar (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carmen Bedia Girb\u00e9s Los esfingol\u00edpidos son una familia lip\u00eddica de compuestos de gran importancia estructural y funcional [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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