{"id":57313,"date":"2007-02-03T00:00:00","date_gmt":"2007-02-03T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/produccion-de-l-carnitina-mediante-cepas-de-escherichia-coli-aplicacion-de-las-ingenierias-metabolica-y-genetica\/"},"modified":"2007-02-03T00:00:00","modified_gmt":"2007-02-03T00:00:00","slug":"produccion-de-l-carnitina-mediante-cepas-de-escherichia-coli-aplicacion-de-las-ingenierias-metabolica-y-genetica","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/procesos-metabolicos\/produccion-de-l-carnitina-mediante-cepas-de-escherichia-coli-aplicacion-de-las-ingenierias-metabolica-y-genetica\/","title":{"rendered":"Producci\u00f3n de l(-)-carnitina mediante cepas de escherichia coli: aplicaci\u00f3n de las ingenier\u00edas metab\u00f3lica y gen\u00e9tica."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Vicente Bernal S\u00e1nchez <\/strong><\/h2>\n<p>En la presente tesis doctoral se ha llevado a cabo la optimizaci\u00f3n de la producci\u00f3n de l(-)-carnitina empleando el metabolismo de compuestos de trimetilamonio de escherichia coli desde los puntos de vista del bioproceso y el metabolismo.  se ha llevado a cabo un an\u00e1lisis sistem\u00e1tico de los principales factores que afectan al proceso de producci\u00f3n de l(-)-carnitina empleando cepas de escherichia coli, estudi\u00e1ndose el efecto que tiene la disponibilidad de aceptores electr\u00f3nicos (tales como ox\u00edgeno molecular o fumarato) tanto en condiciones de operaci\u00f3n en discontinuo como en continuo. El an\u00e1lisis empleando citometr\u00eda de flujo del efecto sobre las c\u00e9lulas nos permiti\u00f3 determinar las respuestas en los niveles de dna, rna y prote\u00ednas, y la integraci\u00f3n de estos datos con la heterogeneidad de las poblaciones celulares. Esto nos permiti\u00f3 analizar de una manera novedosa el modo en el que la configuraci\u00f3n del reactor afecta a la fisiolog\u00eda de las c\u00e9lulas. Adem\u00e1s, se estudi\u00f3 el contenido en dna de las c\u00e9lulas de e. Coli en cultivos continuos, permiti\u00e9ndonos determinar los factores que determinan la estabilizaci\u00f3n gen\u00e9tica de la cepa inmovilizada. Adem\u00e1s, la expresi\u00f3n del metabolismo secundario se coordin\u00f3 con la de las rutas centrales a trav\u00e9s de prote\u00ednas reguladoras generales, permitiendo la evoluci\u00f3n de los pools de cofactores y metabolitos la uni\u00f3n o integraci\u00f3n de ambos metabolismos. El an\u00e1lisis metab\u00f3lico en condiciones de estr\u00e9s revel\u00f3 un aumento en la productividad debido a la permeabilizaci\u00f3n celular y a la activaci\u00f3n de las rutas metab\u00f3licas de generaci\u00f3n de energ\u00eda y precursores. Se clon\u00f3, sobreexpres\u00f3 y caracteriz\u00f3 parcialmente la prote\u00edna caic, revel\u00e1ndose como una ligasa de coa altamente espec\u00edfica. Adem\u00e1s, se construyeron cepas de sobreexpresi\u00f3n y deleci\u00f3n de las actividades caib (coa transferasa) y caic (coa ligasa) estudi\u00e1ndose el efecto de estas modificaciones sobre la producci\u00f3n de l(-)-carnitina y subray<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Producci\u00f3n de l(-)-carnitina mediante cepas de escherichia coli: aplicaci\u00f3n de las ingenier\u00edas metab\u00f3lica y gen\u00e9tica.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Producci\u00f3n de l(-)-carnitina mediante cepas de escherichia coli: aplicaci\u00f3n de las ingenier\u00edas metab\u00f3lica y gen\u00e9tica. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Vicente Bernal S\u00e1nchez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 02\/03\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Manuel Canovas Diaz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: nicholas Lindley <\/li>\n<li>Juan  Manuel Lema rodicio (vocal)<\/li>\n<li>arturo Manjon rubio (vocal)<\/li>\n<li> Lozano teruel Jos\u00e9 Antonio (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Vicente Bernal S\u00e1nchez En la presente tesis doctoral se ha llevado a cabo la optimizaci\u00f3n de la 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