{"id":57684,"date":"2018-03-09T22:45:32","date_gmt":"2018-03-09T22:45:32","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/ubc13-computational-and-experimental-studies-of-its-two-major-interaction-surfaces\/"},"modified":"2018-03-09T22:45:32","modified_gmt":"2018-03-09T22:45:32","slug":"ubc13-computational-and-experimental-studies-of-its-two-major-interaction-surfaces","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/ubc13-computational-and-experimental-studies-of-its-two-major-interaction-surfaces\/","title":{"rendered":"Ubc13: computational and experimental studies of its two major interaction surfaces"},"content":{"rendered":"

Tesis doctoral de Johanna Scheper Sigmund <\/strong><\/h2>\n

Introducci\u00f3n: la ubiquitinaci\u00f3n es una modificacion post-traduccional de las proteinas.Durante mucho tiempo se rey\u00f3 que era una modificaci\u00f3n limitada a se\u00f1alizar para la degradaci\u00f3n de proteinas a trav\u00e9s del proteasoma.Recientemente se ha visto que tambi\u00e9n puede verse implicada en otros procesos celulares tales como el tr\u00e1fico vesicular, la reparaci\u00f3n de dna, la transcripci\u00f3n…Etc. mecanismos de cat\u00e1lisi de la ubiquitina: se da en tres pasos enzim\u00e1ticos catalizados por una e1 o enzima activadora de ubiquitinas, una e2 o enzima conjugadora de ubiquitinas, y una e3 o ligasa de ubiquitinas. Primero la e1 activa la ubiquitina en un mecanismo atp dependiente y una vez \u00e9sta se encuentra activada es transferida a una e2 que seguidamente la conjugar\u00e1 al substrato aportado por la e3. particularidades de la ubiquitinaci\u00f3n por lisina 63: este proceso difiere del can\u00f3nico o estandart porque consiste en la formaci\u00f3n de cadenas de poliubiquitinas unidas por enlaces isospet\u00eddicos entre la glicina 76 de la ubiquitina donadora y la lisina 63 de la ubiquitina aceptora en vez de la lisina en posici\u00f3n 48 que es la que utiliza la ruta can\u00f3nica y que se\u00f1aliza para degradaci\u00f3n por proteasoma.Este tipo de modificaci\u00f3n es llevado a cabo por el heterod\u00edmero ubc13-uev y tiene sus efectos en rutas como la de nf-kb o en reparaci\u00f3n de dna. objetivos del trabajo: 1) dise\u00f1ar un inhibidor del heterod\u00edmero ubc13-uev 2) identificar proteinas que interactuan con ubc13 utilizando an\u00e1lisis de caracter\u00edsticas en com\u00fan de las interacciones observadas. resultados: se realiz\u00f3 un cribaje de una quimioteca de peptoides utilizando la tecnica del doble h\u00edbrido en levadura. De las mezclas de peptoides testadas se pudo deducir 4 posibles peptoides individuales capaces de inhibir la interacci\u00f3n de ubc13 y uev. Estos peptoides fueron utilizados en una selecci\u00f3n computacional para dise\u00f1ar moleculas mas r\u00edgidas que derivaran de ellas y que encajaran bien en la superfic<\/p>\n

 <\/p>\n

Datos acad\u00e9micos de la tesis doctoral \u00abUbc13: computational and experimental studies of its two major interaction surfaces<\/strong>\u00ab<\/h3>\n
    \n
  • T\u00edtulo de la tesis:<\/strong>\u00a0 Ubc13: computational and experimental studies of its two major interaction surfaces <\/li>\n
  • Autor:<\/strong>\u00a0 Johanna Scheper Sigmund <\/li>\n
  • Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n
  • Fecha de lectura de la tesis:<\/strong>\u00a0 26\/03\/2007<\/li>\n<\/ul>\n

     <\/p>\n

    Direcci\u00f3n y tribunal<\/h3>\n
      \n
    • Director de la tesis<\/strong>\n
        \n
      • Timothy Thomson Okatsu<\/li>\n<\/ul>\n<\/li>\n
      • Tribunal<\/strong>\n
          \n
        • Presidente del tribunal: aaron Ciechanover <\/li>\n
        • Manuel Rodr\u00edguez (vocal)<\/li>\n
        • Fernando Albericio palomera (vocal)<\/li>\n
        • mario Menc\u00eda (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n

           <\/p>\n","protected":false},"excerpt":{"rendered":"

          Tesis doctoral de Johanna Scheper Sigmund Introducci\u00f3n: la ubiquitinaci\u00f3n es una modificacion post-traduccional de las proteinas.Durante mucho tiempo se rey\u00f3 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[35],"tags":[127470,1099,127469,41397,127471,13682],"class_list":["post-57684","post","type-post","status-publish","format-standard","hentry","category-ciencias-de-la-vida","tag-aaron-ciechanover","tag-fernando-albericio-palomera","tag-johanna-scheper-sigmund","tag-manuel-rodriguez","tag-mario-mencia","tag-timothy-thomson-okatsu"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/57684","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=57684"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/57684\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=57684"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=57684"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=57684"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}