{"id":57843,"date":"2018-03-09T22:45:40","date_gmt":"2018-03-09T22:45:40","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aproximacional-sistema-cutinola%c2%adtico-de-trichoderma-harzianum-caracterizacion-y-analisis-funcional-del-gen-thcut1-y-del-factor-de-transcripcion-thctf1\/"},"modified":"2018-03-09T22:45:40","modified_gmt":"2018-03-09T22:45:40","slug":"aproximacional-sistema-cutinola%c2%adtico-de-trichoderma-harzianum-caracterizacion-y-analisis-funcional-del-gen-thcut1-y-del-factor-de-transcripcion-thctf1","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/micologia\/aproximacional-sistema-cutinola%c2%adtico-de-trichoderma-harzianum-caracterizacion-y-analisis-funcional-del-gen-thcut1-y-del-factor-de-transcripcion-thctf1\/","title":{"rendered":"Aproximaci\u00f3nal sistema cutinol\u00edtico de trichoderma harzianum. caracterizaci\u00f3n y an\u00e1lisis funcional del gen thcut1 y del factor de transcripci\u00f3n thctf1"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Belen Rubio Perez <\/strong><\/h2>\n<p>El objetivo general de esta tesis doctoral ha sido el estudio del sistema cutinol\u00edtico de trichoderma harzianum. Mediante una aproximaci\u00f3n gen\u00f3mica se han clonado y caracterizado los genes thcut1 y thctf1 de la cepa de biocontrol trichoderma harzianum t34, que presentan una alta similitud con genes relacionados con cutinasas descritos en diversos hongos fitopat\u00f3genos.  el gen thcut1 present\u00f3 una alta similitud con cutinasas de las especies de hongos pyrenopeziza brassicae, botrytis cinerea y monilinia fructicola, y con una lipasa de aspergillus oryzae. Se encuentra como copia \u00fanica en el genoma de t. Harzianum t34, y su expresi\u00f3n se reprime por glucosa y se induce en presencia de aceite de oliva. Para analizar la actividad de thcut1 se recurri\u00f3 a una estrategia de sobreexpresi\u00f3n heter\u00f3loga en la cepa gs115 de la levadura pichia pastoris. La actividad esterasa detectada en los transformantes de pichia que expresan el gen thcut1 confirma la naturaleza ester\u00e1sica de la prote\u00edna thcut1.  por otro lado, el gen thctf1 present\u00f3 una similitud muy alta con un factor de transcripci\u00f3n activador de cutinasas (ctf1\u00edY) descrito en fusarium solani f. Sp. Pisi. Se encuentra tambi\u00e9n como copia \u00fanica en el genoma de t. Harzianum t34, y su expresi\u00f3n no se reprime por glucosa. Para determinar la funci\u00f3n del gen thctf1, se recurri\u00f3 a una estrategia de disrupci\u00f3n g\u00e9nica. La ausencia de producci\u00f3n de dos alquil-pironas, no descritas previamente en el g\u00e9nero trichoderma, en los transformantes interrumpidos y la menor actividad antif\u00fangica de los mismos, demuestran que el factor de transcripci\u00f3n thctf1 est\u00e1 relacionado con la producci\u00f3n de metabolitos secundarios en t. Harzianum t34 y con las habilidades de esta cepa como agente de control biol\u00f3gico.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aproximaci\u00f3nal sistema cutinol\u00edtico de trichoderma harzianum. caracterizaci\u00f3n y an\u00e1lisis funcional del gen thcut1 y del factor de transcripci\u00f3n thctf1<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aproximaci\u00f3nal sistema cutinol\u00edtico de trichoderma harzianum. caracterizaci\u00f3n y an\u00e1lisis funcional del gen thcut1 y del factor de transcripci\u00f3n thctf1 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Belen Rubio Perez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Salamanca<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 30\/03\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Rosa Hermosa Prieto<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francisco Rey iglesias <\/li>\n<li>Carlos Nicolas rodriguez (vocal)<\/li>\n<li>Santiago Gutierrez martin (vocal)<\/li>\n<li>isidro Gonzalez collado (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Belen Rubio Perez El objetivo general de esta tesis doctoral ha sido el estudio del sistema cutinol\u00edtico [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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