{"id":58509,"date":"2018-03-09T22:46:20","date_gmt":"2018-03-09T22:46:20","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-del-promotor-vav1-para-la-expresion-de-genes-en-el-sistema-hematopoyetico-aplicaciones-en-transgenesis-y-terapia-genica\/"},"modified":"2018-03-09T22:46:20","modified_gmt":"2018-03-09T22:46:20","slug":"estudio-del-promotor-vav1-para-la-expresion-de-genes-en-el-sistema-hematopoyetico-aplicaciones-en-transgenesis-y-terapia-genica","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica\/estudio-del-promotor-vav1-para-la-expresion-de-genes-en-el-sistema-hematopoyetico-aplicaciones-en-transgenesis-y-terapia-genica\/","title":{"rendered":"Estudio del promotor vav1 para la expresi\u00f3n de genes en el sistema hematopoy\u00e9tico: aplicaciones en transg\u00e9nesis y terapia g\u00e9nica"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Elena Almarza Novoa <\/strong><\/h2>\n<p>El promotor que regula la expresi\u00f3n del protooncog\u00e9n vav se considera de gran inter\u00e9s debido a que este gen se expresa de forma ubicua en c\u00e9lulas del sistema hematopoy\u00e9tico. La expresi\u00f3n del protooncog\u00e9n vav viene regulada por cinco sitios hipersensibles para dnasa i (hs), siendo el primero de ellos (vavhs1) el promotor proximal. Se ha generado una l\u00ednea de ratones transg\u00e9nicos en cepa pura con fenotipo ly5.1 en el que la expresi\u00f3n del gen marcador deltahcd4 estaba bajo el control del promotor vavhs21\/45. En estos animales se observ\u00f3 la expresi\u00f3n de deltahcd4 en todos los linajes analizados, tanto en sangre perif\u00e9rica como en m\u00e9dula \u00f3sea, incluyendo progenitores primitivos de m\u00e9dula \u00f3sea. Nuestros resultados demuestran por primera vez que el promotor vavhs21\/45 es activo en precursores primitivos con capacidad de repoblaci\u00f3n a largo de plazo. Se ha generado un vector en el que la expresi\u00f3n del gen marcador gfp viene dirigida por el promotor proximal de vav en el contexto de un vector autoinactivante. Se ha observado que la actividad de este promotor es d\u00e9bil y estable en l\u00edneas celulares transducidas con el lentivirus vavhs1. Tambi\u00e9n se ha podido observar que esta d\u00e9bil y estable actividad del promotor vavhs1 se mantiene en c\u00e9ulas hematopoy\u00e9ticas primitivas murinas y humanas in vivo. Estas caracter\u00edsticas se mantuvieron independientemente de la orientaci\u00f3n del promotor, del contexto retroviral o de la presencia\/ausencia de la secuencia wpre. Para demostrar la eficacia terap\u00e9utica del promotor vavhs1 se gener\u00f3 un vector lentiviral autoinactivante que expresaba el gen de anemia de fanconi a (fanca. As\u00ed, y de manera consistente con datos previos, comprobamos que este vector proporcionaba una expresi\u00f3n d\u00e9bil del gen fanca pero que, sin embargo, permit\u00eda la correcci\u00f3n del fenotipo de fanconi que presentaban las c\u00e9lulas deficientes de este gen. En conclusi\u00f3n, nuestros resultados sugieren que tanto vectores lentivirales autoinactivantes<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio del promotor vav1 para la expresi\u00f3n de genes en el sistema hematopoy\u00e9tico: aplicaciones en transg\u00e9nesis y terapia g\u00e9nica<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio del promotor vav1 para la expresi\u00f3n de genes en el sistema hematopoy\u00e9tico: aplicaciones en transg\u00e9nesis y terapia g\u00e9nica <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Elena Almarza Novoa <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/05\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Antonio Bueren Roncero<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: agustin gregorio Zapata gonzalez <\/li>\n<li>Jorge Barquinero manez (vocal)<\/li>\n<li>eugenio Montini (vocal)<\/li>\n<li>Francisco Martin molina (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Elena Almarza Novoa El promotor que regula la expresi\u00f3n del protooncog\u00e9n vav se considera de gran inter\u00e9s [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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