{"id":58677,"date":"2018-03-09T22:46:31","date_gmt":"2018-03-09T22:46:31","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-y-clonaje-molecular-de-protea%c2%adnas-del-polen-de-salsola-kali\/"},"modified":"2018-03-09T22:46:31","modified_gmt":"2018-03-09T22:46:31","slug":"caracterizacion-y-clonaje-molecular-de-protea%c2%adnas-del-polen-de-salsola-kali","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-y-clonaje-molecular-de-protea%c2%adnas-del-polen-de-salsola-kali\/","title":{"rendered":"Caracterizaci\u00f3n y clonaje molecular de prote\u00ednas del polen de salsola kali"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Esther Civantos Mart\u00edn <\/strong><\/h2>\n<p>Introducci\u00f3n     salsola kali es una maleza que pertenece al a familia chenopodiaceae, produce gran cantidad de polen y se la considera la m\u00e1s alerg\u00e9nica de la familia. Se encuentra ampliamente distribuida a lo largo de la costa de europa, norte de \u00e1frica, estados unidos y australia. En espa\u00f1a aparece en la cuenca del ebro y en la zona de alicante, murcia y almer\u00eda.  objetivo     el objetivo de este trabajo es la caracterizaci\u00f3n molecular de algunas prote\u00ednas del polen de salsola kali, entre ellas su ant\u00edgeno mayoritario (42 kda), y el clonaje de una de sus prote\u00ednas alerg\u00e9nicas por reactividad cruzada con chenopodium album.  material y m\u00e9todos     la caracterizaci\u00f3n molecular de las prote\u00ednas del polen de s.Kali se llev\u00f3 a cabo mediante sds-page, electroforesis bidimensional, estudios de desglicosilaci\u00f3n y de prote\u00ednas liposolubles y espectrometr\u00eda de masas. La caracterizaci\u00f3n inmunol\u00f3gica se realiz\u00f3 por medio de inmunodetecciones con sueros de 76 pacientes, densitometrados e inmunodetecci\u00f3n de las prote\u00ednas separadas pro electroforesis bidimenisonal con un pool de sueros y un antisuero de rat\u00f3n frente a la prote\u00edna de 42 kda de s.Kali. Una prote\u00edna de s.Kali se clon\u00f3 y expres\u00f3. Dado que exist\u00eda un bloque de la regi\u00f3n n-terminal de la prote\u00edna de 42 kda de s,kali, se obtuvieron los cebadores utilizando el extremo n-terminal de una prote\u00edna del mismo peso molecular de chenopodium \u00e1lbum, que presenta una identidad inmunol\u00f3gica total con s.Kali. Se hizo una inmunodetecci\u00f3n con los sueros de los pacientes para analizar la reactividad de la prote\u00edna recombinante.  resultados     39 prote\u00ednas distintas se separan por sds-page de las cuales un total de 18 son reconocidas por alguno de los sueros. el extracto presenta tanto prote\u00ednas glicosilados como liposolubles que son reconocidas por la ige de un pool de sueros de pacientes sensibilizados. Mediante la electroforesis bidimensional se obtuvieron prote\u00ednas de distinto<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n y clonaje molecular de prote\u00ednas del polen de salsola kali<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n y clonaje molecular de prote\u00ednas del polen de salsola kali <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Esther Civantos Mart\u00edn <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 28\/05\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Carlos De La Lahoz Navarro<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda  monica De la fuente del rey <\/li>\n<li>isabel Cortegano jimeno (vocal)<\/li>\n<li>Carlos Col\u00e1s sanz (vocal)<\/li>\n<li>ignacio Moneo goiri (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Esther Civantos Mart\u00edn Introducci\u00f3n salsola kali es una maleza que pertenece al a familia chenopodiaceae, produce gran [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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