{"id":59047,"date":"2018-03-09T22:46:53","date_gmt":"2018-03-09T22:46:53","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/cellular-implications-of-alterations-in-potassium-homeostasis-in-saccharomyces-cerevisiae\/"},"modified":"2018-03-09T22:46:53","modified_gmt":"2018-03-09T22:46:53","slug":"cellular-implications-of-alterations-in-potassium-homeostasis-in-saccharomyces-cerevisiae","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/politecnica-de-valencia\/cellular-implications-of-alterations-in-potassium-homeostasis-in-saccharomyces-cerevisiae\/","title":{"rendered":"Cellular implications of alterations in potassium homeostasis in saccharomyces cerevisiae"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Emilie Merchan Stephanie <\/strong><\/h2>\n<p>Los mecanismos de homeostasis de k+ y h+, aunque muy estudiados, siguen siendo procesos conocidos parcialmente. En el caso de la levadura, saccharomyces cerevisiae, la salida de protones de la c\u00e9lula esta regulada por la h+-atpasa pma1p, cuyo funcionamiento depende de la entrada de potasio por medio de los transportadores de alta afinidad trk1 y trk2. Estudios gen\u00e9ticos han establecido que el transportador trk1 esta activado por las quinasas hal4\/sat4 y hal5 e inhibido por las fosfatasas ppz1 y ppz2.  en la relaci\u00f3n con la integridad celular, demostramos que las cepas que carecen de los genes ppz1 y ppz2 muestran un aumento en la turgencia debido a una acumulaci\u00f3n excesiva de potasio, que afecta tanto al tama\u00f1o celular como a la tolerancia a altas concentraciones de este i\u00f3n en el medio externo. Tambi\u00e9n demostramos que el incremento de la turgencia produce un estr\u00e9s constante en la pared celular de estas cepas. Estos fenotipos son dependientes de la presencia de los genes que codifican los transportadores de potasio de alta afinidad, trk1 y trk2 y pueden ser rescatados por la presencia de un estabilizador osm\u00f3tico.  en una segunda parte del trabajo, hemos establecido que trk1 esta localizado en subdominios de la membrana plasm\u00e1tica llamados \u00abrafts\u00bb. Adem\u00e1s, demostramos que ppz1 tambi\u00e9n est\u00e1 localizado en la membrana plasm\u00e1tica, que su localizaci\u00f3n subcelular se ve modulada seg\u00fan los niveles de expresi\u00f3n de trk1 y que ppz1 y trk1 co-inmunoprecipitan in vivo. Por otro lado, trk1 aparece fosforilado tanto in vivo como in vitro y su fosforilaci\u00f3n in vivo est\u00e1 aumentada en mutantes ppz1 ppz2. Este conjunto de datos apoya la hip\u00f3tesis que las fosfatasas ppz son reguladores negativos del transportador de alta afinidad trk1. finalmente, observando la progresi\u00f3n del ciclo celular, hemos observado que los mutantes ppz1ppz2 son mas sensibles a agentes que da\u00f1an el dna, presentan un retraso en el desarrollo de la fase s del ciclo celular, y tienen una m<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Cellular implications of alterations in potassium homeostasis in saccharomyces cerevisiae<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Cellular implications of alterations in potassium homeostasis in saccharomyces cerevisiae <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Emilie Merchan Stephanie <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de Valencia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 15\/06\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Lynne Yenush<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: jos\u00e9 Ramos ruiz <\/li>\n<li>Mar\u00eda Molina mart\u00edn (vocal)<\/li>\n<li>Juan  Carlos Igual Garc\u00eda (vocal)<\/li>\n<li>joaqu\u00edn Ari\u00f1o carmona (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Emilie Merchan Stephanie Los mecanismos de homeostasis de k+ y h+, aunque muy estudiados, siguen siendo procesos [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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